ArticleApplied microbiology and biotechnology2020
Comparative genomics of Streptococcus parauberis: new target for molecular identification of serotype III.
Article in Applied microbiology and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Serotype distribution and antibiogram ofMicrobiology spectrum · 2023Article
- Complete Genome Sequences of Streptococcus parauberis Serotype Ia, Ib/Ic, and II Isolates from Diseased Olive Flounder in South Korea.Microbiology resource announcements · 2023Article
- Virulence and antibiotic-resistance genes in Enterococcus faecalis associated with streptococcosis disease in fish.Scientific reports · 2023Article
- Genomic characterization of Tenacibaculum maritimum O-antigen gene cluster and development of a multiplex PCR-based serotyping scheme.Transboundary and emerging diseases · 2022Article
- Transcriptome analysis unveils survival strategies of Streptococcus parauberis against fish serum.PloS one · 2021Article
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2 authors.
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Abstract
This paper describes the predicted structure for the cps loci involved in capsule biosynthesis for Streptococcus parauberis serotypes III, IV, and V. Based on the specific serotype regions I, II, and III, a multiplex PCR protocol (mPCR) was designed to differentiate the main serotypes causing fish diseases. A real-time PCR method (qPCR) is also described to identify S. parauberis of serotype III in bacterial cultures and fish tissues. In silico and in vitro analyses revealed that both methods have a 100% specificity. The mPCR assay was optimized for the detection of S. parauberis strains of subtypes Ia (amplicon size 213 bp), subtypes Ib and Ic (both amplicon size 303 bp), serotype II (amplicon size 403 bp), and serotype III (amplicon size 130 bp) from bacterial cultures. The qPCR assay was optimized for the identification and quantification of S. parauberis serotype III strains in bacterial cultures and fish tissues. This assay achieved a sensitivity of 2.67 × 10
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