ArticleExperimental eye research2020
Segmental differences found in aqueous angiographic-determined high - and low-flow regions of human trabecular meshwork.
Article in Experimental eye research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Aqueous Angiography-Guided Bent Ab Interno Needle Goniectomy in High Versus Low Aqueous Humor Outflow Regions in Primary Open Angle Glaucoma: A Pilot Randomized Controlled Trial.Journal of glaucoma · 2025Trial
- Segmental flow responses in glaucomatous trabecular meshwork cells.Biomaterials advances · 2026Article
- Machine-Learning-Based Automated Schlemm's Canal Volumetric Segmentation for Optical Coherence Tomography.Chemical & biomedical imaging · 2026Article
- Cholinergic Improvement of Low-Flow Segmental Aqueous Humor Outflow in Humans.Ophthalmology science · 2026Article
- Investigation of two pore types in Schlemm's canal inner wall endothelium in flow-type areas using serial block-face scanning electron microscopy.Experimental eye research · 2025Article
- Robotic Visible-Light Optical Coherence Tomography Visualizes Segmental Schlemm's Canal Anatomy and Segmental Pilocarpine Response.Investigative ophthalmology & visual science · 2025Article
- Aqueous Humor Angiography.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Segmental Aqueous Humor Outflow.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches.Investigative ophthalmology & visual science · 2024Article
- Robotic Visible-Light Optical Coherence Tomography Visualizes Segmental Schlemm's Canal Anatomy and Segmental Pilocarpine Response.bioRxiv : the preprint server for biology · 2024Article
- Digital spatial profiling of segmental outflow regions in trabecular meshwork reveals a role for ADAM15.PloS one · 2024Article
- Differences in Outflow Facility Between Angiographically Identified High- Versus Low-Flow Regions of the Conventional Outflow Pathways in Porcine Eyes.Investigative ophthalmology & visual science · 2023Article
- Segmental outflow dynamics in the trabecular meshwork of living mice.Experimental eye research · 2022Article
- Pulsatile Trabecular Meshwork Motion: An Indicator of Intraocular Pressure Control in Primary Open-Angle Glaucoma.Journal of clinical medicine · 2022Article
- The role of Vitamin D3 in ocular fibrosis and its therapeutic potential for the glaucomatous trabecular meshwork.Frontiers in ophthalmology · 2022Review
- Aqueous humour outflow imaging: seeing is believing.Eye (London, England) · 2021Review
- Greater Outflow Facility Increase After Targeted Trabecular Bypass in Angiographically Determined Low-low Regions.Ophthalmology. GlaucomaArticle
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Authors and funding
7 authors.
Funding
Abstract
This work sought to compare aqueous angiographic segmental patterns with bead-based methods which directly visualize segmental trabecular meshwork (TM) tracer trapping. Additionally, segmental protein expression differences between aqueous angiographic-derived low- and high-outflow human TM regions were evaluated. Post-mortem human eyes (One Legacy and San Diego eye banks; n = 15) were perfused with fluorescent tracers (fluorescein [2.5%], indocyanine green [0.4%], and/or fluorescent microspheres). After angiographic imaging (Spectralis HRA+OCT; Heidelberg Engineering), peri-limbal low- and high-angiographic flow regions were marked. Aqueous angiographic segmental outflow patterns were similar to fluorescent microsphere TM trapping segmental patterns. TM was dissected from low- and high-flow areas and processed for immunofluorescence or Western blot and compared. Versican expression was relatively elevated in low-flow regions while MMP3 and collagen VI were relatively elevated in high-flow regions. TGF-β2, thrombospondin-1, TGF-β receptor1, and TGF-β downstream proteins such as α-smooth muscle actin were relatively elevated in low-flow regions. Additionally, fibronectin (FN) levels were unchanged, but the EDA isoform (FN-EDA) that is associated with fibrosis was relatively elevated in low-flow regions. These results show that segmental aqueous angiographic patterns are reflective of underlying TM molecular characteristics and demonstrate increased pro-fibrotic activation in low-flow regions. Thus, we provide evidence that aqueous angiography outflow visualization, the only tracer outflow imaging method available to clinicians, is in part representative of TM biology.
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