ArticlePlant biotechnology journal2020
Cytosolic malate dehydrogenase 4 modulates cellular energetics and storage reserve accumulation in maize endosperm.
Article in Plant biotechnology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 42 citations in OpenAlex.
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- Enzymatic and proteomic changes in resistant and susceptible cacao cultivars reveal distinct response mechanisms toFrontiers in plant science · 2026Article
- RepA Protein of Citrus Chlorotic Dwarf-Associated Virus Impairs Perinuclear Chloroplast Clustering Induced by Lemon Chloroplast Malate Dehydrogenase.Molecular plant pathology · 2025Article
- Polymerization of beneficial plant height QTLs to develop superior lines which can achieving hybrid performance levels.Molecular breeding : new strategies in plant improvement · 2025Article
- Phenotypic characterization and genetic mapping of the semi-dwarf mutant sdw9 in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Genome-Wide Identification and Expression Analysis of Malate Dehydrogenase Gene Family in Sweet Potato and Its Two Diploid Relatives.International journal of molecular sciences · 2023Article
- Effects of Vine Water Status on Malate Metabolism and γ-Aminobutyric Acid (GABA) Pathway-Related Amino Acids in Marselan (Foods (Basel, Switzerland) · 2023Article
- OsMDH12: A Peroxisomal Malate Dehydrogenase Regulating Tiller Number and Salt Tolerance in Rice.Plants (Basel, Switzerland) · 2023Article
- The genetic architecture of prolificacy in maize revealed by association mapping and bulk segregant analysis.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Multi-omic characterization of the maize GPI synthesis mutant gwt1 with defects in kernel development.BMC plant biology · 2023Article
- Novel insights into maize (Zea mays) development and organogenesis for agricultural optimization.Planta · 2023Review
- A Systemic Investigation of Genetic Architecture and Gene Resources Controlling Kernel Size-Related Traits in Maize.International journal of molecular sciences · 2023Article
- Genome-Wide Identification ofPlants (Basel, Switzerland) · 2022Article
- Metabolic Profiling of Sugars and Organic Acids, and Expression Analyses of Metabolism-Associated Genes in Two Yellow-Peel Pitaya Species.Plants (Basel, Switzerland) · 2022Article
- Physiological Responses and Metabonomics Analysis of Male and FemaleFrontiers in plant science · 2022Article
- Maize kernel development.Molecular breeding : new strategies in plant improvement · 2021Review
- Cytosolic malate dehydrogenase 4 modulates cellular energetics and storage reserve accumulation in maize endosperm.Plant biotechnology journal · 2020Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytosolic malate dehydrogenase (MDH) is a key enzyme that regulates the interconversion between malate and oxaloacetate (OAA). However, its role in modulating storage compound accumulation in maize endosperm is largely unknown. Here, we characterized a novel naturally occurring maize mdh4-1 mutant, which produces small, opaque kernels and exhibits reduced starch but enhanced lysine content. Map-based cloning, functional complementation and allelism analyses identified ZmMdh4 as the causal gene. Enzymatic assays demonstrated that ZmMDH4 predominantly catalyses the conversion from OAA to malate. In comparison, the activity of the mutant enzyme, which lacks one glutamic acid (Glu), was completed abolished, demonstrating that the Glu residue was essential for ZmMDH4 function. Knocking down ZmMdh4 in vivo led to a substantial metabolic shift towards glycolysis and a dramatic disruption in the activity of the mitochondrial complex I, which was correlated with transcriptomic alterations. Taken together, these results demonstrate that ZmMdh4 regulates the balance between mitochondrial respiration and glycolysis, ATP production and endosperm development, through a yet unknown feedback regulatory mechanism in mitochondria.
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