ArticleFrontiers in bioengineering and biotechnology2020
Consensus Design of an Evolved High-Redox Potential Laccase.
Article in Frontiers in bioengineering and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Article
- Purification, characterization and three-dimensional structure prediction of multicopper oxidase Laccases from Trichoderma lixii FLU1 and Talaromyces pinophilus FLU12.Scientific reports · 2024Article
- Efficiency of thermostable purified laccase isolated from Physisporinus vitreus for azo dyes decolorization.World journal of microbiology & biotechnology · 2024Article
- Thermal Stability Enhancement of L-Asparaginase fromFoods (Basel, Switzerland) · 2023Article
- Protein Engineering Approaches to Enhance Fungal Laccase Production inInternational journal of molecular sciences · 2021Article
- Ancestral Resurrection and Directed Evolution of Fungal Mesozoic Laccases.Applied and environmental microbiology · 2020Article
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Authors and funding
4 authors.
Funding
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Abstract
Among the broad repertory of protein engineering methods that set out to improve stability, consensus design has proved to be a powerful strategy to stabilize enzymes without compromising their catalytic activity. Here, we have applied an in-house consensus method to stabilize a laboratory evolved high-redox potential laccase. Multiple sequence alignments were carried out and computationally refined by applying relative entropy and mutual information thresholds. Through this approach, an ensemble of 20 consensus mutations were identified, 18 of which were consensus/ancestral mutations. The set of consensus variants was produced in
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