ArticleBone & joint research2020
Recalcitrant methicillin-resistant
Article in Bone & joint research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- A methodological approach to improve extracellular staphylococcal clearance in internalization assays using bone-associated host-cell models of orthopedic infections.Frontiers in cellular and infection microbiology · 2026Article
- Post-fluoroquinolone treatment molecular events and nutrient availability modulatebioRxiv : the preprint server for biology · 2025Article
- Baohuoside I targets SaeR as an antivirulence strategy to disrupt MRSA biofilm formation and pathogenicity.NPJ biofilms and microbiomes · 2025Article
- Antibody-antibiotic conjugate targeted therapy for orthopedic implant-associated intracellular S. aureus infections.Journal of advanced research · 2024Article
- Advances in the targeted theragnostics of osteomyelitis caused by Staphylococcus aureus.Archives of microbiology · 2024Review
- The surgical management of fracture-related infection. Surgical strategy selection and the need for early surgical intervention.Journal of orthopaedics · 2024Article
- An Orthopedic Perspective on Diabetic Foot Attack - A Case Report.Journal of orthopaedic case reports · 2024Article
- Parathyroid hormone therapy improves MRSA-infected fracture healing in a murine diabetic model.Frontiers in cellular and infection microbiology · 2023Article
- Repurposing Inhibitors of Phosphoinositide 3-kinase as Adjuvant Therapeutics for Bacterial Infections.Frontiers in antibiotics · 2023Article
- Concurrent targeting of glycolysis in bacteria and host cell inflammation in septic arthritis.EMBO molecular medicine · 2022Article
- Extended local release and improved bacterial eradication by adding rifampicin to a biphasic ceramic carrier containing gentamicin or vancomycin.Bone & joint research · 2022Article
- Rifampicin restores extracellular organic matrix formation and mineralization of osteoblasts after intracellularBone & joint research · 2022Article
- The intersection of fracture healing and infection: Orthopaedics research society workshop 2021.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Review
- Platelets mediate trained immunity against bone and joint infections in a mouse model.Bone & joint research · 2022Article
- Treating 'Septic' With Enhanced Antibiotics and 'Arthritis' by Mitigation of Excessive Inflammation.Frontiers in cellular and infection microbiology · 2022Article
- The osteoblast secretome inFrontiers in immunology · 2022Review
- In vitro antibiotic activity against intraosteoblastic Staphylococcus aureus: a narrative review of the literature.The Journal of antimicrobial chemotherapy · 2021Review
- Surgical management of the acute severely infected diabetic foot - The 'infected diabetic foot attack'. An instructional review.Journal of clinical orthopaedics and trauma · 2021Article
- Dual therapeutic targeting of intra-articular inflammation and intracellular bacteria enhances chondroprotection in septic arthritis.Science advances · 2021Article
- An AntisenseAntibiotics (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
aimsTo characterize the intracellular penetration of osteoblasts and osteoclasts by methicillin-resistant
methodsTime-lapse confocal microscopy was used to analyze the interaction of MRSA strain USA300 with primary murine osteoblasts and osteoclasts. The effects of early and delayed antibiotic treatments on intracellular and extracellular bacterial colony formation and cell death were quantified. We tested the effects of cefazolin, gentamicin, vancomycin, tetracycline, rifampicin, and ampicillin, as well as agents used in surgical preparation and irrigation.
resultsMRSA infiltrated bone-resident cells within 15 to 30 minutes. Penetration was most effectively prevented with early (i.e. 30 minutes) antibiotic administration. The combined administration of rifampicin with other antibiotics potentiated their protective effects against MRSA-induced cytotoxicity and most significantly reduced extracellular bacterial bioburden. Gentamicin-containing compounds were most effective in reducing intracellular MRSA bioburden. Of the surgical preparation agents evaluated, betadine reduced in vitro MRSA growth to the greatest extent.
conclusionThe standard of care for open fractures involves debridement and antibiotics within the first six hours of injury but does not account for the window in which bacteria penetrate cells. Antibiotics must be administered as early as possible after injury or prior to incision to prevent intracellular infestation. Rifampicin can potentiate the capacity of antibiotic regimens to reduce MRSA-induced cytotoxicity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.