ArticleJournal of clinical microbiology2020
Evaluation of Serological Tests for Detection of Antibodies against Lumpy Skin Disease Virus.
Article in Journal of clinical microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Comparison of multiple antigens for use in indirect ELISAs for detection of anti-capripoxvirus antibodies in sheep, goats, and cattle.Journal of clinical microbiology · 2026Article
- Assessment of humoral immune responses elicited by an inactivated lumpy skin disease vaccine in cattle: a preliminary study.The Journal of veterinary medical science · 2026Article
- Diagnostic approach, vaccination and treatment priorities for Lumpy Skin Disease in cattle: lessons from Balkan epidemics to Western Europe re-emergence (2025).Veterinary research communications · 2026Review
- Recombinant P32 and B5R proteins of lumpy skin disease virus as candidate antigens for serodiagnostic applications.Veterinary research communications · 2026Article
- Development and Validation of a Population Assay for the Seroprevalence of Lumpy Skin Disease.Microorganisms · 2026Article
- Development of a monoclonal antibody-based competitive ELISA for serological detection of lumpy skin disease virus.Frontiers in veterinary science · 2026Article
- Article
- Review
- Development of a double-antigen sandwich ELISA for rapid and accurate detection of antibodies against Capripoxvirus.Microbiology spectrum · 2025Article
- Epidemiology of lumpy skin disease in Northeast India and a new method for rapid field diagnosis.AMB Express · 2025Article
- Progress in diagnostic methods and vaccines for lumpy skin disease virus: a path towards understanding the disease.Veterinary research communications · 2025Review
- Evaluation of antibody titer and associated risk factors of goat pox vaccine against lumpy skin disease in crossbred buffaloes.Veterinary research communications · 2025Article
- Lumpy skin disease: A growing threat to the global livestock industry.Open veterinary journal · 2025Review
- Serological response to lumpy skin disease in recovered and clinically healthy vaccinated and unvaccinated cattle of Bangladesh.Frontiers in veterinary science · 2025Article
- Two proteins, one goal: ELISAs based on p32 and L1R for LSDV antibodies detection.Frontiers in veterinary science · 2025Article
- Evidence of natural lumpy skin disease virus (LSDV) infection and genetic characterization of LSDV strains from water buffaloes (Bubalus bubalis) in India.Archives of virology · 2024Article
- Development of a competitive ELISA based on the LSDV A33 antigen.Virology journal · 2024Article
- Evaluation of longitudinal passive immunity transfer against lumpy skin disease virus in calves by different serological methods.Veterinary research communications · 2024Article
- Persistence of passive immunity in calves receiving colostrum from cows vaccinated with a live attenuated lumpy skin disease vaccine and the performance of serological tests.Frontiers in veterinary science · 2024Article
- Emergency vaccination of cattle against lumpy skin disease: Evaluation of safety, efficacy, and potency of MEVACVeterinary research communications · 2023Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lumpy skin disease (LSD) is an emerging, transboundary viral pox disease affecting cattle of all ages and breeds. The serological assay for monitoring immunity following vaccination is a virus neutralization test (VNT/OIE) that determines the neutralization index (NI). The first validated enzyme-linked immunosorbent assay (ELISA; IDVet) has become commercially available, facilitating large-scale serosurveillance for LSD. Although the VNT is labor intensive and time consuming, it is still the recommended test by the OIE. Thus, in this study, we modified the virus neutralization test by employing Madin-Darby bovine kidney (MDBK) cells. The qualitative results obtained with the modified method were compared to the qualitative results obtained by VNT/OIE and ELISA. We used blood sera received within a surveillance program for LSD in 2018. In total, 291 serum samples were tested using VNT/MDBK and ELISA. Of 291 samples, 80 samples were tested by VNT/OIE and used for comparison of the performances between VNT/MDBK and VNT/OIE. The compatibility of results obtained by VNT/MDBK and VNT/OIE resulted in a kappa index of 0.9 with overall proportion agreement of 0.96. Agreement between VNT/MDBK and VNT/OIE was achieved in 56 positive and 21 negative samples. The compatibility of results obtained by ELISA and VNT/MDBK were compared on 291 samples in total and resulted in a kappa index 0.834 with overall proportion agreement of 0.955. Agreement between ELISA and VNT/MDBK was achieved in 238 positive and 40 negative samples. The results obtained demonstrated a strong correlation between VNT/MDBK and the other two methods, indicating the suitability of VNT/MDBK for the detection of the LSD virus-specific neutralizing antibodies.
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