ArticleEuropean journal of medical research2020
Effect of glutathione-stabilized silver nanoparticles on expression of las I and las R of the genes in Pseudomonas aeruginosa strains.
Article in European journal of medical research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 22 citations in OpenAlex.
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- Antibiofilm activity of biosynthesized silver and copper nanoparticles using Streptomyces S29.AMB Express · 2023Article
- Comparative assessment of green and chemically synthesized glutathione capped silver nanoparticles for antioxidant, mosquito larvicidal and eco-toxicological activities.Scientific reports · 2023Article
- An investigation on glutathione derived from spinach and red cabbage leaves and their effects of adding to meat patties.Saudi journal of biological sciences · 2023Article
- Investigation the effects of silver nanoparticles and gold nanoparticles on expression ofIranian journal of microbiology · 2022Article
- Knocking down Pseudomonas aeruginosa virulence by oral hypoglycemic metformin nano emulsion.World journal of microbiology & biotechnology · 2022Article
- Effect of Titanium Dioxide Nanoparticles on the Expression of Efflux Pump and Quorum-Sensing Genes in MDRAntibiotics (Basel, Switzerland) · 2021Article
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2 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundBiofilm formation is regarded as a significant factor in the establishment of infections caused by Pseudomonas aeruginosa. P. aeruginosa is one of the most important causes of nosocomial infections. Today silver nanoparticles (Ag-NPs) are used as antimicrobials due to their well-known, chemical, biological, and physical properties. Exposure to nanoparticles could inhibit colonization of new bacteria onto the biofilm.
methodsIn the present work, the green synthesis of Ag-NPs was performed using the alcoholic extract of Eucalyptus camaldulensis. Ag-NPs and glutathione-stabilized silver nanoparticles (GSH-Ag-NPs) were characterized using X-ray diffraction (XRD), dynamic light scattering (DLS), scanning electron microscope (SEM), and carbon, nitrogen, and hydrogen (CNH) and Fourier transform infrared spectroscopy (FTIR) techniques were applied to investigate the structure of the modified nanoparticles. Then, the antimicrobial and antibiofilm potential of the prepared Ag-NPs and GSH-Ag-NPs against P. aeruginosa strains was evaluated using microbroth dilution method and their effects on the expression of las I and las R genes.
resultsIn this study, a total of 50 P. aeruginosa isolates were recovered from clinical samples. According to the results, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) value of Ag-NPs against P. aeruginosa was determined to be 512-256 μg/ml, respectively, while the MIC and MBC value of GS-Ag-NPs against P. aeruginosa clinical strains was determined in a range of 128-256 μg/ml and 256-512 μg/ml, respectively. The mean expression level in las R, las I genes in P. aeruginosa strains treated with ½ MIC of Ag-NPs was decreased by -5.7 and -8fold, respectively. The mean expression levels of las R, las I genes in P. aeruginosa strains treated with ½ MIC of GS-Ag-NPs were decreased by -8.7 and -10fold, respectively (P < 0.05).
conclusionsThe results of our study showed that Ag-NPs and GS-Ag-NPs are highly effective against P. aeruginosa strains. Moreover, this study also proves the promising potential of using nanoparticles as anti-biofilm formation and antibacterial agents.
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