Evidence map›Paper›PMID 32434565›Full record

ArticleGenome biology2020

Gapless assembly of maize chromosomes using long-read technologies.

Jianing Liu, Arun S Seetharam, Kapeel Chougule, Shujun Ou, Kyle W Swentowsky, Jonathan I Gent, Victor Llaca, Margaret R Woodhouse, Nancy Manchanda, Gernot G Presting and 8 more

Abstract readEvaluation Study
In one paragraph

Article in Genome biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.

0numbers the graph read from it
0cells of the map it votes in
72citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

72 citing papers in PubMed.

  1. Article
  2. The evolutionary genomics of meiotic drive.Molecular biology and evolution · 2026
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  19. Investigating thebioRxiv : the preprint server for biology · 2024
    Article
  20. Article

12 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Jianing LiuDepartment of Genetics, University of Georgia, Athens, GA, 30602, USA.
Arun S SeetharamGenome Informatics Facility, Iowa State University, Ames, IA, 50011, USA.
Kapeel ChouguleCold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724, USA.
Shujun OuDepartment of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, IA, 50011, USA.
Kyle W SwentowskyDepartment of Plant Biology, University of Georgia, Athens, GA, 30602, USA.
Jonathan I GentDepartment of Plant Biology, University of Georgia, Athens, GA, 30602, USA.
Victor LlacaCorteva Agriscience™, 8325 NW 62nd Ave, Johnston, IA, 50131, USA.
Margaret R WoodhouseUSDA-ARS Corn Insects and Crop Genetics Research Unit, Ames, IA, 50011, USA.
Nancy ManchandaDepartment of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, IA, 50011, USA.
Gernot G PrestingMolecular Biosciences and Bioengineering, University of Hawaii, Honolulu, HI, 96822, USA.
David A KudrnaArizona Genomics Institute, School of Plant Sciences, University of Arizona, Tucson, AZ, 85721, USA.
Magdy AlabadyDepartment of Plant Biology, University of Georgia, Athens, GA, 30602, USA.
Candice N HirschDepartment of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN, 55108, USA.
Kevin A FenglerCorteva Agriscience™, 8325 NW 62nd Ave, Johnston, IA, 50131, USA.
Doreen WareCold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724, USA.
Todd P MichaelInformatics Department, J. Craig Venter Institute, La Jolla, CA, USA.
Matthew B HuffordDepartment of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, IA, 50011, USA.
R Kelly DaweDepartment of Genetics, University of Georgia, Athens, GA, 30602, USA. kdawe@uga.edu.ORCID 0000-0003-3407-4553

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Creating gapless telomere-to-telomere assemblies of complex genomes is one of the ultimate challenges in genomics. We use two independent assemblies and an optical map-based merging pipeline to produce a maize genome (B73-Ab10) composed of 63 contigs and a contig N50 of 162 Mb. This genome includes gapless assemblies of chromosome 3 (236 Mb) and chromosome 9 (162 Mb), and 53 Mb of the Ab10 meiotic drive haplotype. The data also reveal the internal structure of seven centromeres and five heterochromatic knobs, showing that the major tandem repeat arrays (CentC, knob180, and TR-1) are discontinuous and frequently interspersed with retroelements.

Indexed as

Chromosomes, PlantGenome, PlantGenomicsPhysical Chromosome MappingZea maysGapless assemblyKnob structureLong-read technologyMaize genomeMeiotic drive

Identifiers

PMID32434565
PMCPMC7238635

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.