ArticleScientific reports2020
Microbiota in vitro modulated with polyphenols shows decreased colonization resistance against Clostridioides difficile but can neutralize cytotoxicity.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.
- Biological Mechanisms of Polyphenols againstInfectious disorders drug targets · 2025Pooled it
- Miniature bioreactor arrays for modeling functional and structural dysbiosis in inflammatory bowel disease.Gut microbes · 2026Article
- Enabling next-generation anaerobic cultivation through biotechnology to advance functional microbiome research.Nature biotechnology · 2025Review
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- A complementary metaproteomic approach to interrogate microbiome cultivated from clinical colon biopsies.Proteomics · 2024Article
- Review
- The microbial metabolite urolithin A reducesmSystems · 2024Article
- Clostridioides difficile Biofilm.Advances in experimental medicine and biology · 2024Article
- Protective effects of blueberries on vascular function: A narrative review of preclinical and clinical evidence.Nutrition research (New York, N.Y.) · 2023Review
- MiniBioReactor Array (MBRA)JAC-antimicrobial resistance · 2022Article
- Disordered Intestinal Microbial Communities DuringFrontiers in cellular and infection microbiology · 2022Article
- Review
- Severity of Experimental Autoimmune Uveitis Is Reduced by Pretreatment with Live ProbioticCells · 2020Article
Corrections and comments
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Authors and funding
5 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While the knowledge on gut microbiota - C. difficile interactions has improved over the years, the understanding of the underlying mechanisms providing colonization resistance as well as preventative measures against the infection remain incomplete. In this study the antibiotic clindamycin and polyphenol extracts from pomegranate and blueberries were used individually and in combination to modulate fecal microbial communities in minibioreactor arrays (MBRA). Modulated communities were inoculated with C. difficile (ribotype 027). Subsequent 7-day periodical monitoring included evaluation of C. difficile growth and activity of toxins TcdA and TcdB as well as analysis of MBRA bacterial community structure (V3V4 16 S metagenomics). Polyphenols affected multiple commensal bacterial groups and showed different synergistic and antagonistic effects in combination with clindamycin. Exposure to either clindamycin or polyphenols led to the loss of colonization resistance against C. difficile. The successful growth of C. difficile was most significantly correlated with the decrease in Collinsella and Lachnospiraceae. Additionally, we demonstrated that Clostridium sporogenes decreased the activity of both C. difficile toxins TcdA and TcdB. The feature was shown to be common among distinct C. sporogenes strains and could potentially be applicable as a non-antibiotic agent for the alleviation of C. difficile infection.
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Registered trials
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