ArticleFrontiers in bioengineering and biotechnology2020
Robot Cookies - Plant Cell Packs as an Automated High-Throughput Screening Platform Based on Transient Expression.
Article in Frontiers in bioengineering and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- A high-throughput heterologous expression platform for plant synthetic biology based on Arabidopsis suspension cells.Journal of experimental botany · 2026Article
- Non-invasive imaging of defence responses in plants.Nature communications · 2026Article
- Nicotiana benthamiana's Responses to Agroinfiltration, a Treasure Grove of New Avenues to Improve Protein Yields in Plant Molecular Farming.Plant biotechnology journal · 2026Review
- Streamlined molecular farming of plant virus therapeutics for space flight and other low-resource environments.NPJ science of plants · 2026Article
- Constitutive Promoters Functional in Plant, Fungal, and Bacterial Hosts.ACS synthetic biology · 2025Article
- Harnessing Transient Expression Systems with Plant Viral Vectors for the Production of Biopharmaceuticals inInternational journal of molecular sciences · 2025Review
- Transient expression of full-length and mature nattokinase in Nicotiana benthamiana reveals early necrosis from full-length form and functional activity of the mature enzyme.Frontiers in plant science · 2025Article
- A hybrid pathway for self-sustained luminescence.Science advances · 2024Article
- An improved pathway for autonomous bioluminescence imaging in eukaryotes.Nature methods · 2024Article
- Design, optimization, production and activity testing of recombinant immunotoxins expressed in plants and plant cells for the treatment of monocytic leukemia.Bioengineered · 2023Article
- Antimicrobial Peptides: The Production of Novel Peptide-Based Therapeutics in Plant Systems.Life (Basel, Switzerland) · 2023Review
- Risk assessment and bioburden evaluation of Agrobacterium tumefaciens-mediated transient protein expression in plants using the CaMV35S promoter.BMC biotechnology · 2023Article
- Manufacture and evaluation of a HER2-positive breast cancer immunotoxin 4D5Fv-PE25.Microbial cell factories · 2023Article
- Plant-based biopharmaceutical engineering.Nature reviews bioengineering · 2023Review
- Systematic Comparison of Plant Promoters inInternational journal of molecular sciences · 2022Article
- The transient expression of recombinant proteins in plant cell packs facilitates stable isotope labelling for NMR spectroscopy.Plant biotechnology journal · 2022Article
- Integrating plant molecular farming and materials research for next-generation vaccines.Nature reviews. Materials · 2022Review
- Optimising expression and extraction of recombinant proteins in plants.Frontiers in plant science · 2022Review
- Contributions of the international plant science community to the fight against human infectious diseases - part 1: epidemic and pandemic diseases.Plant biotechnology journal · 2021Review
- Synthetic biology of plant natural products: From pathway elucidation to engineered biosynthesis in plant cells.Plant communications · 2021Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The high-throughput screening of recombinant protein expression is advantageous during early process development because it allows the identification of optimal expression constructs and process conditions. Simple screening platforms based on microtiter plates are available for microbes and animal cells, but this was not possible for plants until the development of plant cell packs (PCPs), also known as "cookies," which provide a versatile and scalable screening tool for recombinant protein production. PCPs are prepared from plant cell suspension cultures by removing the medium and molding the biomass. PCPs can be cast into 96-well plates for high-throughput screening, but the manual handling effort currently limits the throughput to ∼500 samples per day. We have therefore integrated the PCP method with a fully automated laboratory liquid-handling station. The "robot cookies" can be prepared and infiltrated with
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.