ArticleFrontiers in genetics2020
Cryopreservation Induces Alterations of miRNA and mRNA Fragment Profiles of Bull Sperm.
Article in Frontiers in genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 44 citations in OpenAlex.
- Heat Stress and the Bull Sperm Transcriptome: Molecular Mechanisms, Fertility, and Epigenetic Inheritance.Animals : an open access journal from MDPI · 2026Review
- Intraepididymal platelet-rich plasma improves semen cryoresistance via antioxidant, lipid and molecular modulation during the non-breeding season in rams.Veterinary research communications · 2026Article
- Silent cells? Potential for context-dependent gene expression in mature sperm.Proceedings. Biological sciences · 2025Review
- The Relationship between The Expression of SpermInternational journal of fertility & sterility · 2024Article
- Effects of Slow Freezing and Vitrification of Human Semen on Post-Thaw Semen Quality and miRNA Expression.International journal of molecular sciences · 2024Article
- Effect of cryopreservation and semen extender on extracellular vesicles isolated from bull semen.Frontiers in veterinary science · 2024Article
- Cryopreservation Induces Acetylation of Metabolism-Related Proteins in Boar Sperm.International journal of molecular sciences · 2023Article
- Cryostress induces fragmentation and alters the abundance of sperm transcripts associated with fertilizing competence and reproductive processes in buffalo.Cell and tissue research · 2023Article
- Integrated transcriptomics and proteomics assay identifies the role of FCGR1A in maintaining sperm fertilization capacity during semen cryopreservation in sheep.Frontiers in cell and developmental biology · 2023Article
- Identification and analysis of differentially expressed (DE) circRNA in epididymis of yak and cattleyak.Frontiers in veterinary science · 2023Article
- Effect of Sperm Cryopreservation in Farm Animals Using Nanotechnology.Animals : an open access journal from MDPI · 2022Review
- The future of assessing bull fertility: Can the 'omics fields identify usable biomarkers?†.Biology of reproduction · 2022Review
- The cryopreservation process induces alterations in proteins associated with bull sperm quality: The equilibration process could be a probable critical control point.Frontiers in endocrinology · 2022Article
- Feline microRNAome in ovary and testis: Exploration ofFrontiers in genetics · 2022Article
- miR-301a-5p RegulatesGenes · 2021Article
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Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although cryopreservation of bull semen is widely used commercially in the livestock breeding industry, cryopreservation results in low fertility of bull sperm. As an important regulatory factor, the alteration of small non-coding RNA (sncRNA) profile during cryopreservation of bull sperm is not yet completely known. In the present study, we sequenced sncRNAs of frozen and fresh sperm to study the link of alteration of the sncRNA profiles (particularly in miRNAs and mRNA fragments) with low sperm fertility caused by cryopreservation. We identified 55 miRNAs and 526 mRNA fragments differentially expressed (DE) between frozen and fresh sperm. Subsequently, the functional analysis revealed that targeted genes of DE miRNAs in sperm had roles in the fertilization, ATP, and apoptosis. Instead, targeted genes of DE miRNAs in cow metaphase II oocyte were significantly enriched in the MAPK signaling pathway, autophagy-animal pathway, and mitophagy-animal pathway. Additionally, biological processes of DNA repair, spermatid development, response to temperature stimulus, and cellular response to DNA damage stimulus were enriched by mRNA fragments. In conclusion, we found that DE miRNAs or DE mRNA fragments in cryopreservation may influence the fertility of sperm, these findings will provide the reference to improve the cryopreservation technology of bull semen.
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