ArticleFrontiers in plant science2020
Complex Trait Loci in Maize Enabled by CRISPR-Cas9 Mediated Gene Insertion.
Article in Frontiers in plant science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 79 citations in OpenAlex.
- Genome-Edited Maize Expressing Two Native Genes Confers Broad-Spectrum Resistance to Northern Corn Leaf Blight.Molecular plant pathology · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- The future of genome editing in plants.Nature plants · 2025Review
- It's Complicated: Why Are There So Few Commercially Successful Crop Varieties Engineered for Disease Resistance?Molecular plant pathology · 2025Review
- Development of efficient targeted insertion mediated by CRISPR-Cas12a and homology-directed repair in maize.Frontiers in genome editing · 2025Article
- Recent advances in site-specific transgene insertion into the maize genome using recombinases and genome editing endonucleases.Frontiers in plant science · 2025Review
- Soybean genomics research community strategic plan: A vision for 2024-2028.The plant genome · 2024Article
- Cas12a-mediated gene targeting by sequential transformation strategy in Arabidopsis thaliana.BMC plant biology · 2024Article
- Precise integration of large DNA sequences in plant genomes using PrimeRoot editors.Nature biotechnology · 2024Article
- Application of multiple sgRNAs boosts efficiency of CRISPR/Cas9-mediated gene targeting in Arabidopsis.BMC biology · 2024Article
- Leveraging the sugarcane CRISPR/Cas9 technique for genetic improvement of non-cultivated grasses.Frontiers in plant science · 2024Review
- Simple promotion of Cas9 and Cas12a expression improves gene targeting via an all-in-one strategy.Frontiers in plant science · 2024Article
- Biotechnological Tools to Elucidate the Mechanism of Plant and Nematode Interactions.Plants (Basel, Switzerland) · 2023Review
- Review
- Leaf transformation for efficient random integration and targeted genome modification in maize and sorghum.Nature plants · 2023Article
- Insights into the molecular mechanisms of CRISPR/Cas9-mediated gene targeting at multiple loci in Arabidopsis.Plant physiology · 2022Article
- Target Lines forInternational journal of molecular sciences · 2022Article
- Back to the wild: mining maize (Zea mays L.) disease resistance using advanced breeding tools.Molecular biology reports · 2022Article
- Efficient gene targeting in soybean using Ochrobactrum haywardense-mediated delivery of a marker-free donor template.Plant physiology · 2022Article
- A Broad Review of Soybean Research on the Ongoing Race to Overcome Soybean Cyst Nematode.Biology · 2022Review
Corrections and comments
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Authors and funding
22 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Modern maize hybrids often contain biotech and native traits. To-date all biotech traits have been randomly inserted in the genome. Consequently, developing hybrids with multiple traits is expensive, time-consuming, and complex. Here we report using CRISPR-Cas9 to generate a complex trait locus (CTL) to facilitate trait stacking. A CTL consists of multiple preselected sites positioned within a small well-characterized chromosomal region where trait genes are inserted. We generated individual lines, each carrying a site-specific insertion landing pad (SSILP) that was targeted to a preselected site and capable of efficiently receiving a transgene via recombinase-mediated cassette exchange. The selected sites supported consistent transgene expression and the SSILP insertion had no effect on grain yield. We demonstrated that two traits residing at different sites within a CTL can be combined via genetic recombination. CTL technology is a major step forward in the development of multi-trait maize hybrids.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.