Evidence map›Paper›PMID 32431693›Full record

ArticleFrontiers in immunology2020

Production of Autoreactive Heavy Chain-Only Antibodies in Systemic Lupus Erythematosus.

Shu Xu, Hong Yang, Yue Zhuo, Yangsheng Yu, Hongyan Liao, Song Li, Yinshi Yue, Kaihong Su, Zhixin Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Shu XuState Key Laboratory of Biotherapy, Sichuan University, Chengdu, China.
Hong YangState Key Laboratory of Biotherapy, Sichuan University, Chengdu, China.
Yue ZhuoHealth Management Center, Institute of Health Management, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
Yangsheng YuDepartment of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, United States.
Hongyan LiaoDepartment of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.
Song LiDepartment of Medical Oncology, Cancer Center, Qilu Hospital of Shandong University, Jinan, China.
Yinshi YueDepartment of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, United States.
Kaihong SuEducation-Microbiology/Immunology, Department of Medical Education, California University of Science and Medicine, San Bernardino, CA, United States.
Zhixin ZhangHealth Management Center, Institute of Health Management, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
Sichuan University · CNUniversity of Electronic Science and Technology of China · CNUniversity of Nebraska Medical Center · USCalifornia State University, San Bernardino · USQilu Hospital of Shandong University · CN

Funding

Novel Neutrophil Autoantigens in Systemic Lupus ErythematosusR01AR059351 · NIAMS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI SU, KAIHONG · 2011 to 2015
$1.5M
Accumulation of VH replacement products in HIV patientsR56AI098576 · NIAID · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI ZHANG, ZHIXIN · 2012 to 2012
$453k
Contribution of VH Replacement to B Cell Anti-HIV Immune ResponseR21AI073174 · NIAID · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI ZHANG, ZHIXIN · 2007 to 2008
$363k
NIAID NIH HHS R21 AI073174NIAID NIH HHS R56 AI098576NIAMS NIH HHS R01 AR059351
6 · The paper itself

Abstract

Systemic lupus erythematosus (SLE) is characterized by the overproduction of high-affinity autoreactive antibodies. Here, we show that more than 65.8% of 222 recombinant antibodies derived from 8 SLE patients can be secreted as heavy chain-only antibodies (HCAbs) when expressed in HEK-293T cells. The secretion of HCAbs follows the conventional endoplasmic reticulum-Golgi apparatus pathway, despite triggering a weaker unfolded protein response (UPR). Many of the purified SLE HCAbs remain autoreactive and have an even higher affinity for dsDNA, Sm, nucleosome, and cardiolipin than HCAbs from healthy individuals. Extended analyses of the CDR3 region and the heavy chain variable (VH) region of HCAb F3 show that the VH region is responsible for IgH secretion, while the CDR3 region determines its reactivity. Such a high frequency of HCAb secretion cannot fully concur with our current understanding of antibody assembly and secretion. The presence of a large proportion of autoreactive HCAbs in SLE reveals a novel mechanism for the generation of autoreactive antibodies in lupus.

Indexed as

AdultAmino AcidsAntibody AffinityAutoantibodiesCardiolipinsDNAFemaleHEK293 CellsHumansImmunoglobulin Heavy ChainsImmunoglobulin Variable RegionLupus Erythematosus, SystemicMaleMiddle AgedNucleosomesRecombinant ProteinsAmino AcidsAutoantibodiesCardiolipinsDNAImmunoglobulin Heavy ChainsImmunoglobulin Variable RegionNucleosomesRecombinant Proteinsautoreactive antibodyB cellheavy chain antibodyplasmablastpolyreactive antibodysomatic hypermutation

Identifiers

PMID32431693
PMCPMC7214812
OpenAlexW3021935029

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.