Evidence map›Paper›PMID 32431461›Full record

ArticleMolecular biology2020

A Recombinant Rotavirus Antigen Based on the Coat Protein of Alternanthera Mosaic Virus.

E M Ryabchevskaya, E A Evtushenko, M V Arkhipenko, E K Donchenko, N A Nikitin, J G Atabekov, O V Karpova

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

E M Ryabchevskaya1Department of Virology, Faculty of Biology, Moscow State University, 119234 Moscow, Russia.
E A Evtushenko1Department of Virology, Faculty of Biology, Moscow State University, 119234 Moscow, Russia.
M V Arkhipenko1Department of Virology, Faculty of Biology, Moscow State University, 119234 Moscow, Russia.
E K Donchenko1Department of Virology, Faculty of Biology, Moscow State University, 119234 Moscow, Russia.
N A Nikitin1Department of Virology, Faculty of Biology, Moscow State University, 119234 Moscow, Russia.
J G Atabekov1Department of Virology, Faculty of Biology, Moscow State University, 119234 Moscow, Russia.
O V Karpova1Department of Virology, Faculty of Biology, Moscow State University, 119234 Moscow, Russia.
Lomonosov Moscow State University · RUFederal Center Research Fundamentals of Biotechnology · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Thanks to their strong immunostimulating properties and safety for humans, plant viruses represent an appropriate basis for the design of novel vaccines. The coat protein of Alternanthera mosaic virus can form virus-like particles that are stable under physiological conditions and have adjuvant properties. This work presents a recombinant human rotavirus A antigen based on the epitope of rotavirus structural protein VP6, using Alternanthera mosaic virus coat protein as a carrier. An expression vector containing the gene of Alternanthera mosaic virus (MU strain) coat protein fused to the epitope of rotavirus protein VP6 was designed. Immunoblot analysis showed that the chimeric protein was effectively recognized by commercial polyclonal antibodies to rotavirus and therefore is a suitable candidate for development of a vaccine prototype. Interaction of the chimeric recombinant protein with the native coat protein of Alternanthera mosaic virus and its RNA resulted in the formation of ribonucleoprotein complexes that were recognized by anti-rotavirus antibodies.

Indexed as

plant virusespotexvirusrotavirus infectionvaccinesvirus-like particles

Identifiers

PMID32431461
PMCPMC7222081
OpenAlexW3021791985

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.