ArticleMicrobial cell factories2020
Key factors affecting ammonium production by an Azotobacter vinelandii strain deregulated for biological nitrogen fixation.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 53 citations in OpenAlex.
- Coupling bacterial nitrogen fixation to yeast whole-cell biocatalysis enables amine production using air as the sole nitrogen source.Microbial cell factories · 2026Article
- Overcoming urea and nitrate inhibition of biological nitrogen fixation in Azotobacter vinelandii versus natural tolerance in Gluconacetobacter diazotrophicus.World journal of microbiology & biotechnology · 2026Article
- Characterising complex metabolic responses in an engineered, cross-feeding microbial co-culture using quantitative proteomics.New biotechnology · 2026Article
- Article
- The Role of Plant Growth-Promoting Bacteria in Soil Restoration: A Strategy to Promote Agricultural Sustainability.Microorganisms · 2025Review
- Azotobacter vinelandii as a Nitrogen-Negative Chassis for Bio-Oil and Bio-Wax Production of Heterologous and Native Lipids.MicrobiologyOpen · 2025Article
- Enhanced Urea Production in the Diazotroph Azotobacter vinelandii as a Means of Stable Nitrogen Biofertiliser Production.Microbial biotechnology · 2025Article
- Nitrogen stable isotope fractionation by biological nitrogen fixation reveals cellular nitrogenase is diffusion limited.PNAS nexus · 2025Article
- A deoxyviolacein-based transposon insertion vector for pigmented tracer studies.MicrobiologyOpen · 2024Article
- Precision control of ammonium release in Azotobacter vinelandii.Microbial biotechnology · 2024Article
- Searching for Chemical Agents Suppressing Substrate Microbiota in White-Rot Fungi Large-Scale Cultivation.Microorganisms · 2024Article
- Competitive fitness and stability of ammonium-excreting Azotobacter vinelandii strains in the soil.Applied microbiology and biotechnology · 2024Article
- Scripting a new dialogue between diazotrophs and crops.Trends in microbiology · 2024Review
- Enhanced extracellular ammonium release in the plant endophyteMicrobiology spectrum · 2024Article
- Microalgal and Nitrogen-Fixing Bacterial Consortia: From Interaction to Biotechnological Potential.Plants (Basel, Switzerland) · 2023Review
- Systematic identification of endogenous strong constitutive promoters from the diazotrophic rhizosphere bacterium Pseudomonas stutzeri DSM4166 to improve its nitrogenase activity.Microbial cell factories · 2023Article
- Molecular Mechanism and Agricultural Application of the NifA-NifL System for Nitrogen Fixation.International journal of molecular sciences · 2023Review
- Engineered Nanoparticles, Natural Nanoclay and Biochar, as Carriers of Plant-Growth Promoting Bacteria.Nanomaterials (Basel, Switzerland) · 2022Article
- Gluconacetobacter diazotrophicus Gene Fitness during Diazotrophic Growth.Applied and environmental microbiology · 2022Article
- Plant Growth-Promoting Bacterial Consortia as a Strategy to Alleviate Drought Stress inMicroorganisms · 2022Article
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3 authors at 1 institution in 1 country.
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Abstract
backgroundThe obligate aerobe Azotobacter vinelandii is a model organism for the study of biological nitrogen fixation (BNF). This bacterium regulates the process of BNF through the two component NifL and NifA system, where NifA acts as an activator, while NifL acts as an anti-activator based on various metabolic signals within the cell. Disruption of the nifL component in the nifLA operon in a precise manner results in a deregulated phenotype that produces levels of ammonium that far surpass the requirements within the cell, and results in the release of up to 30 mM of ammonium into the growth medium. While many studies have probed the factors affecting growth of A. vinelandii, the features important to maximizing this high-ammonium-releasing phenotype have not been fully investigated.
resultsIn this work, we report the effect of temperature, medium composition, and oxygen requirements on sustaining and maximizing elevated levels of ammonium production from a nitrogenase deregulated strain. We further investigated several pathways, including ammonium uptake through the transporter AmtB, which could limit yields through energy loss or futile recycling steps. Following optimization, we compared sugar consumption and ammonium production, to attain correlations and energy requirements to drive this process in vivo. Ammonium yields indicate that between 5 and 8% of cellular protein is fully active nitrogenase MoFe protein (NifDK) under these conditions.
conclusionsThese findings provide important process optimization parameters, and illustrate that further improvements to this phenotype can be accomplished by eliminating futile cycles.
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