ArticleBMC oral health2020
Development and application of a 3D periodontal in vitro model for the evaluation of fibrillar biomaterials.
Article in BMC oral health, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.
- Root Cementum Molecular Structure and Its Role in Maintaining Oral Health-Systematic Review.International journal of molecular sciences · 2025Pooled it
- Transgenic high expression of EphB4 in canine periodontal ligament stem cells modulates osteogenic differentiation and migration in cPDLSCs.Biomedical engineering online · 2026Article
- Cytotoxicity in vitro assay in 3D vs. 2D L929 cell cultures - comparative analysis of the response to the latex extracts.PloS one · 2026Article
- Bone Graft Paste Nanohydroxyapatite Chitosan-Gelatin (nHA/KG) for Periodontal Regeneration: Study on Three-Dimensional Cell Culture.European journal of dentistry · 2025Article
- Tannic Acid-Enhanced Gelatin-Based Composite Hydrogel as a Candidate for Canine Periodontal Regeneration.Gels (Basel, Switzerland) · 2025Article
- Smart injectable hydrogels for periodontal regeneration: Recent advancements in biomaterials and biofabrication strategies.Materials today. Bio · 2025Review
- New Insights in Hydrogels for Periodontal Regeneration.Journal of functional biomaterials · 2023Review
- PreclinicalFrontiers in bioengineering and biotechnology · 2023Review
- Proteomic and single-cell analysis shed new light on the anti-inflammatory role of interferonβ in chronic periodontitis.Frontiers in pharmacology · 2023Article
- Biomaterials for Periodontal Regeneration.Dental clinics of North America · 2022Review
- The Effect of Diabetes Mellitus on IGF Axis and Stem Cell Mediated Regeneration of the Periodontium.Bioengineering (Basel, Switzerland) · 2021Review
- The recent advances in scaffolds for integrated periodontal regeneration.Bioactive materials · 2021Review
- Current Trends in In Vitro Modeling to Mimic Cellular Crosstalk in Periodontal Tissue.Advanced healthcare materials · 2021Review
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundPeriodontitis is a chronic inflammation of the tooth supporting structures that finally can lead to tooth loss. As chronic periodontitis is associated with systemic diseases multiple approaches have been followed to support regeneration of the destructed tissue. But very few materials are actually used in the clinic. A new and promising group of biomaterials with advantageous biomechanical properties that have the ability to support periodontal regeneration are self-assembling peptides (SAP). However, there is still a lack of 3D periodontal models that can evaluate the migration potential of such novel materials.
methodsAll experiments were performed with primary human periodontal ligament fibroblasts (HPLF). Migration capacity was assessed in a three-dimensional model of the human periodontal ligament by measuring the migration distance of viable cells on coated (Enamel Matrix Protein (EMP), P11-4, collagen I) or uncoated human dentin. Cellular metabolic activity on P11-4 hydrogels was assessed by a metabolic activity assay. Deposition of ECM molecules in a P11-4 hydrogel was visualized by immunostaining of collagen I and III and fibrillin I.
resultsThe 3D periodontal model was feasible to show the positive effect of EMP for periodontal regeneration. Subsequently, self-assembling peptide P11-4 was used to evaluate its capacity to support regenerative processes in the 3D periodontal model. HPLF coverage of the dentin surface coated with P11-4 increased significantly over time, even though delayed compared to EMP. Cell viability increased and inclusion of ECM proteins into the biomaterial was shown.
conclusionThe presented results indicate that the 3D periodontal model is feasible to assess periodontal defect coverage and that P11-4 serves as an efficient supporter of regenerative processes in the periodontal ligament. CLINICAL RELEVANCE: The establishment of building-block synthetic polymers offers new opportunities for clinical application in dentistry. Self-assembling peptides represent a new generation of biomaterials as they are able to respond dynamically to the changing environment of the biological surrounding. Especially in the context of peri-implant disease prevention and treatment they enable the implementation of new concepts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.