ArticleBMC plant biology2020
Over-expression of a γ-tocopherol methyltransferase gene in vitamin E pathway confers PEG-simulated drought tolerance in alfalfa.
Article in BMC plant biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 33 citations in OpenAlex.
- A century of vitamin E research: The innovative journey from basic biology to synthetic bio-manufacturing.Journal of integrative plant biology · 2026Review
- Forage Crop Research in the Modern Age.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Advances in basic biology of alfalfa (Horticulture research · 2025Article
- An alternative splicing caused by a natural variation in BnaC02.VTE4 gene affects vitamin E and glucosinolate content in rapeseed (Brassica napus L.).Plant biotechnology journal · 2025Article
- Vitamin E in Plants: Biosynthesis Pathways, Biofortification Strategies, and Regulatory Dynamics.International journal of molecular sciences · 2025Review
- Enhancing Food Security via selecting Superior Camelina (Camelina sativa L.) parents: a positive approach incorporating pheno-morphological traits, fatty acids composition, and Tocopherols Content.BMC plant biology · 2025Article
- Understanding abiotic stress in alfalfa: physiological and molecular perspectives on salinity, drought, and heavy metal toxicity.Frontiers in plant science · 2025Review
- Dynamic identification of candidate genes associated with higher tocopherol biosynthesis inFrontiers in plant science · 2025Article
- Ameliorating effect of zinc on water transport in rice plants under saline-sodic stress.Frontiers in plant science · 2025Article
- Anticancer Potential of Tocopherols-Containing Plants and Semi-Synthetic Tocopherols.Plants (Basel, Switzerland) · 2024Review
- A developmental gradient reveals biosynthetic pathways to eukaryotic toxins in monocot geophytes.Cell · 2024Article
- Genotypic variation of tocopherol content in a representative genetic population and genome-wide association study on tocopherol in rapeseed (Molecular breeding : new strategies in plant improvement · 2023Article
- Comparative Transcriptomic and Physiological Analyses Reveal Key Factors for Interstocks to Improve Grafted Seedling Growth in Tangor.International journal of molecular sciences · 2023Article
- Effect of Overexpression ofInternational journal of molecular sciences · 2022Article
- Identification of Genomic Loci and Candidate Genes Related to Seed Tocopherol Content in Soybean.Plants (Basel, Switzerland) · 2022Article
- Type-A response regulators negatively mediate heat stress response by altering redox homeostasis in Arabidopsis.Frontiers in plant science · 2022Article
- Vitamin E synthesis and response in plants.Frontiers in plant science · 2022Review
- Functional Differentiation ofFrontiers in plant science · 2022Article
- Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Extensive Variation in Drought-Induced Gene Expression Changes Between Loblolly Pine Genotypes.Frontiers in genetics · 2021Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundα-Tocopherol is one of the most important vitamin E components present in plant. α-Tocopherol is a potent antioxidant, which can deactivate photoproduced reactive oxygen species (ROS) and prevent lipids from oxidation when plants suffer drought stress. γ-Tocopherol methyltransferase (γ-TMT) catalyzes the formation of α-tocopherol in the tocopherol biosynthetic pathway. Our previous studies showed that over-expression of γ-TMT gene can increase the accumulation of α-tocopherol in alfalfa (Medicago sativa). However, whether these transgenic plants confer increased drought tolerance and the underlying mechanism are still unknown.
resultsIn the present study, we further evaluate transgenic alfalfa lines, and found that over-expression of MsTMT led to an increase in α-tocopherol and total tocopherol level in the transgenic lines compared with the control plant. It was revealed that drought tolerance of the transgenic alfalfa was remarkably increased, with alleviated oxidative damage and accumulation of more osmolytic substances. The stomatal development in transgenic plants was significantly inhibited on both sides of leaves, which may be resulted from the repression of MsSPCHLESS (MsSPCH) gene. The reduced stomatal density of transgenic plants contributes to a lower stomatal conductance and higher water use efficiency (WUE). Moreover, both RNA-seq and qRT-PCR analyses indicate that regulatory mechanism of MsTMT in drought involved in both ABA-dependent and ABA-independent pathways.
conclusionOur results suggest that MsTMT gene plays a positive role in regulating alfalfa response to PEG-simulated drought stress, which might involve complex mechanisms, including ROS scavenging system, stomatal development and multiple phytohormone signaling pathways. This study will broaden our view on the function of γ-TMT gene and provide new strategy for genetic engineering in alfalfa breeding.
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