ArticleMethods and protocols2020
The Influence of DNA Extraction and Lipid Removal on Human Milk Bacterial Profiles.
Article in Methods and protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Antimicrobial Resistant Factors inInternational journal of genomics · 2026Article
- The Association Between Maternal Diet and the Human Milk Microbiome: A Review of Evidence and Methodological Challenges.Microorganisms · 2025Review
- Metagenomic Applications to Herbivore Gut Microbiomes: A Comprehensive Review of Microbial Diversity and Host Interactions.Animals : an open access journal from MDPI · 2025Review
- Evaluating the Potential of Korean Mudflat-DerivedFood science of animal resources · 2024Article
- Analysis of microbial composition and sharing in low-biomass human milk samples: a comparison of DNA isolation and sequencing techniques.ISME communications · 2023Article
- Improvement and Validation of a Genomic DNA Extraction Method for Human Breastmilk.Methods and protocols · 2023Article
- Microbial Profiling of Biltong Processing Using Culture-Dependent and Culture-Independent Microbiome Analysis.Foods (Basel, Switzerland) · 2023Article
- Insufficient Evidence of a Breastmilk Microbiota at Six-Weeks Postpartum: A Pilot Study.Nutrients · 2023Article
- Comparison of the Effectiveness of Four Commercial DNA Extraction Kits on Fresh and Frozen Human Milk Samples.Methods and protocols · 2022Article
- Metagenomic Sequencing for Microbial DNA in Human Samples: Emerging Technological Advances.International journal of molecular sciences · 2022Review
- Contrasting Diversity and Composition of Human Colostrum Microbiota in a Maternal Cohort With Different Ethnic Origins but Shared Physical Geography (Island Scale).Frontiers in microbiology · 2022Article
- Bovine Milk Microbiota: Comparison among Three Different DNA Extraction Protocols To Identify a Better Approach for Bacterial Analysis.Microbiology spectrum · 2021Article
- Article
- Elucidating biofilm diversity on water lily leaves through 16S rRNA amplicon analysis: Comparison of four DNA extraction kits.Applications in plant sciences · 2021Article
- Evaluation of full-length nanopore 16S sequencing for detection of pathogens in microbial keratitis.PeerJ · 2021Article
- Effect of storage, temperature, and extraction kit on the phylogenetic composition detected in the human milk microbiota.MicrobiologyOpen · 2021Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Culture-independent molecular techniques have advanced the characterization of environmental and human samples including the human milk (HM) bacteriome. However, extraction of high-quality genomic DNA that is representative of the bacterial population in samples is crucial. Lipids removal from HM prior to DNA extraction is common practice, but this may influence the bacterial population detected. The objective of this study was to compare four commercial DNA extraction kits and lipid removal in relation to HM bacterial profiles. Four commercial DNA extraction kits, QIAamp
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