ArticleBiofabrication2020
The effect of BMP-mimetic peptide tethering bioinks on the differentiation of dental pulp stem cells (DPSCs) in 3D bioprinted dental constructs.
Article in Biofabrication, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The growth factor multimodality on treating human dental mesenchymal stem cells: a systematic review.BMC oral health · 2024Pooled it
- Hydrogel-based strategies for dentin-pulp complex regeneration: a comprehensive review.Cell and tissue banking · 2026Review
- Physical and Mechanical Characterisation of 3D-Bioprinted Hydrogels for Dental Applications: A Scoping Review.Gels (Basel, Switzerland) · 2026Review
- Modern Drug Delivery Platforms Based on Photocrosslinkable Hydrogels (PCHs) in Dentistry: From Material Characteristics to Clinical Applications-A Review.Pharmaceuticals (Basel, Switzerland) · 2026Review
- Bioengineered Teeth and Regenerative Strategies: Advances in Tissue Engineering and Stem Cell Applications.Annals of biomedical engineering · 2026Review
- Biofabrication for complex tissue regeneration: a scoping review of translational gaps in craniomaxillofacial reconstruction.Frontiers in oral health · 2026Review
- Three-Dimensional Models of the Dental Pulp: Bridging Fundamental Biology and Regenerative Therapy.International journal of molecular sciences · 2025Review
- Evolving transdermal therapeutics: a review on self-dissolving polymeric microneedlesRSC advances · 2025Review
- Optimizing methods for regenerative endodontics.Regenerative medicine · 2025Review
- The comprehensive progress of tooth regeneration from the tooth development to tissue engineering and clinical application.Cell regeneration (London, England) · 2025Review
- Combinatorial strategy for engineering cartilage and bone microtissues using microfluidic cell-laden microgels.Biofabrication · 2025Article
- Hydrogels in Oral Disease Management: A Review of Innovations in Drug Delivery and Tissue Regeneration.Medical science monitor : international medical journal of experimental and clinical research · 2025Review
- Biomimetic materials: a promising strategy for periodontal tissue engineering and regeneration.Frontiers in bioengineering and biotechnology · 2025Review
- Strategies of functionalized GelMA-based bioinks for bone regeneration: Recent advances and future perspectives.Bioactive materials · 2024Review
- Recent Advances in Scaffolds for Guided Bone Regeneration.Biomimetics (Basel, Switzerland) · 2024Review
- The 3-dimensional printing for dental tissue regeneration: the state of the art and future challenges.Frontiers in bioengineering and biotechnology · 2024Review
- In Vitro and In Vivo Biological Assessments of 3D-Bioprinted Scaffolds for Dental Applications.International journal of molecular sciences · 2023Review
- Biopolymers and Their Application in Bioprinting Processes for Dental Tissue Engineering.Pharmaceutics · 2023Review
- Review
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Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The goal of this study was to use 3D bioprinting technology to create a bioengineered dental construct containing human dental pulp stem cells (hDPSCs). To accomplish this, we first developed a novel bone morphogenetic protein (BMP) peptide-tethering bioink formulation and examined its rheological properties, its printability, and the structural stability of the bioprinted construct. Second, we evaluated the survival and differentiation of hDPSCs in the bioprinted dental construct by measuring cell viability, proliferation, and gene expression, as well as histological and immunofluorescent analyses. Our results showed that the peptide conjugation into the gelatin methacrylate-based bioink formulation was successfully performed. We determined that greater than 50% of the peptides remained in the bioprinted construct after three weeks in vitro cell culture. Human DPSC viability was >90% in the bioprinted constructs immediately after the printing process. Alizarin Red staining showed that the BMP peptide construct group exhibited the highest calcification as compared to the growth medium, osteogenic medium, and non-BMP peptide construct groups. In addition, immunofluorescent and quantitative reverse transcription-polymerase chain reaction analyses showed robust expression of dentin sialophosphoprotein and osteocalcin in the BMP peptide dental constructs. Together, these results strongly suggested that BMP peptide-tethering bioink could accelerate the differentiation of hDPSCs in 3D bioprinted dental constructs.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.