ArticleChemistry (Weinheim an der Bergstrasse, Germany)2020
Chemical Profiling of A-to-I RNA Editing Using a Click-Compatible Phenylacrylamide.
Article in Chemistry (Weinheim an der Bergstrasse, Germany), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- ENDONUCLEASE V: FROM TRANSCRIPTOME REGULATOR TO CHEMICAL BIOLOGY TOOL.ChemistryEurope · 2026Article
- Codon decoding by orthogonal tRNAs interrogates theFrontiers in genetics · 2024Article
- Forced Intercalation Peptide Nucleic Acid Probes for the Detection of an Adenosine-to-Inosine Modification.ACS omega · 2023Article
- A Fluorescent Reverse-Transcription Assay to Detect Chemical Adducts on RNA.Biochemistry · 2022Article
- Harnessing Nature's Molecular Recognition Capabilities to Map and Study RNA Modifications.Accounts of chemical research · 2022Article
- Labeling and sequencing nucleic acid modifications using bio-orthogonal tools.RSC chemical biology · 2022Review
- Protein-based molecular recognition tools for detecting and profiling RNA modifications.Current opinion in structural biology · 2021Review
- Bioorthogonal chemistry-based RNA labeling technologies: evolution and current state.Chemical communications (Cambridge, England) · 2020Article
- A-to-I RNA Editing in Cancer: From Evaluating the Editing Level to Exploring the Editing Effects.Frontiers in oncology · 2020Review
- Advances in Detection Methods for A-to-I RNA Editing.Wiley interdisciplinary reviews. RNAReview
- Experimental and Computational Tools for Mapping Circular RNA Modifications.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Straightforward methods for detecting adenosine-to-inosine (A-to-I) RNA editing are key to a better understanding of its regulation, function, and connection with disease. We address this need by developing a novel reagent, N-(4-ethynylphenyl)acrylamide (EPhAA), and illustrating its ability to selectively label inosine in RNA. EPhAA is synthesized in a single step, reacts rapidly with inosine, and is "click"-compatible, enabling flexible attachment of fluorescent probes at editing sites. We first validate EPhAA reactivity and selectivity for inosine in both ribonucleosides and RNA substrates, and then apply our approach to directly monitor in vitro A-to-I RNA editing activity using recombinant ADAR enzymes. This method improves upon existing inosine chemical-labeling techniques and provides a cost-effective, rapid, and non-radioactive approach for detecting inosine formation in RNA. We envision this method will improve the study of A-to-I editing and enable better characterization of RNA modification patterns in different settings.
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Registered trials
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