Evidence map›Paper›PMID 32427907›Full record

ArticleNature communications2020

Precise phylogenetic analysis of microbial isolates and genomes from metagenomes using PhyloPhlAn 3.0.

Francesco Asnicar, Andrew Maltez Thomas, Francesco Beghini, Claudia Mengoni, Serena Manara, Paolo Manghi, Qiyun Zhu, Mattia Bolzan, Fabio Cumbo, Uyen May and 8 more

Open access · goldAbstract readEvaluation Study
In one paragraph

Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 484 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
484citing papers in PubMed, 1 pooled it
41.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

484 citing papers in PubMed, 1 synthesis or guideline pooled it, 852 citations in OpenAlex.

  1. Pooled it
  2. Trial
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  8. InJournal of bacteriology · 2026
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  12. Moraxella species isolated from blood cultures in Europe (MORAXEu): a multicentre study of epidemiology and antimicrobial susceptibility with complementary phylogenomic analysis of publicly available genomes.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026
    Article
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  18. The Oral Microbiome of King Richard III of England.American journal of biological anthropology · 2026
    Article
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  20. Article

424 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 4 institutions in 3 countries.

Francesco AsnicarDepartment CIBIO, University of Trento, Trento, Italy.ORCID http://orcid.org/0000-0003-3732-1468
Andrew Maltez ThomasDepartment CIBIO, University of Trento, Trento, Italy.ORCID http://orcid.org/0000-0001-5789-3354
Francesco BeghiniDepartment CIBIO, University of Trento, Trento, Italy.ORCID http://orcid.org/0000-0002-8105-9607
Claudia MengoniDepartment CIBIO, University of Trento, Trento, Italy.
Serena ManaraDepartment CIBIO, University of Trento, Trento, Italy.ORCID http://orcid.org/0000-0001-6371-528X
Paolo ManghiDepartment CIBIO, University of Trento, Trento, Italy.
Qiyun ZhuDepartment of Pediatrics, University of California San Diego, La Jolla, CA, USA.
Mattia BolzanDepartment CIBIO, University of Trento, Trento, Italy.ORCID http://orcid.org/0000-0001-8539-9468
Fabio CumboDepartment CIBIO, University of Trento, Trento, Italy.ORCID http://orcid.org/0000-0003-2920-5838
Uyen MayDepartment of Electrical and Computer Engineering, University of California San Diego, La Jolla, CA, USA.
Jon G SandersDepartment of Pediatrics, University of California San Diego, La Jolla, CA, USA.ORCID http://orcid.org/0000-0001-6077-4014
Moreno ZolfoDepartment CIBIO, University of Trento, Trento, Italy.ORCID http://orcid.org/0000-0001-6661-4046
Evguenia KopylovaDepartment of Pediatrics, University of California San Diego, La Jolla, CA, USA.
Edoardo PasolliDepartment CIBIO, University of Trento, Trento, Italy.
Rob KnightDepartment of Pediatrics, University of California San Diego, La Jolla, CA, USA.ORCID http://orcid.org/0000-0002-0975-9019
Siavash MirarabDepartment of Electrical and Computer Engineering, University of California San Diego, La Jolla, CA, USA.ORCID http://orcid.org/0000-0001-5410-1518
Curtis HuttenhowerDepartment of Biostatistics, Harvard T. H. Chan School of Public Health, Boston, MA, USA.ORCID http://orcid.org/0000-0002-1110-0096
Nicola SegataDepartment CIBIO, University of Trento, Trento, Italy. nicola.segata@unitn.it.ORCID http://orcid.org/0000-0002-1583-5794
University of Trento · ITUniversity of California San Diego · USBroad Institute · USCornell University · US

Funding

Exploiting Public Metagenomic Data to Uncover Cancer-Microbiome RelationshipsR01CA230551 · NCI · GRADUATE SCHOOL OF PUBLIC HEALTH AND HEALTH POLICY · PI Levi Waldron · 2020 to 2026
$3.9M
Exploiting public metagenomic data to uncover cancer-microbiome relationshipsU01CA230551 · NCI · GRADUATE SCHOOL OF PUBLIC HEALTH AND HEALTH POLICY · PI WALDRON, LEVI · 2019 to 2019
$464k
NCI NIH HHS R01 CA230551NCI NIH HHS U01 CA230551
6 · The paper itself

Abstract

Microbial genomes are available at an ever-increasing pace, as cultivation and sequencing become cheaper and obtaining metagenome-assembled genomes (MAGs) becomes more effective. Phylogenetic placement methods to contextualize hundreds of thousands of genomes must thus be efficiently scalable and sensitive from closely related strains to divergent phyla. We present PhyloPhlAn 3.0, an accurate, rapid, and easy-to-use method for large-scale microbial genome characterization and phylogenetic analysis at multiple levels of resolution. PhyloPhlAn 3.0 can assign genomes from isolate sequencing or MAGs to species-level genome bins built from >230,000 publically available sequences. For individual clades of interest, it reconstructs strain-level phylogenies from among the closest species using clade-specific maximally informative markers. At the other extreme of resolution, it scales to large phylogenies comprising >17,000 microbial species. Examples including Staphylococcus aureus isolates, gut metagenomes, and meta-analyses demonstrate the ability of PhyloPhlAn 3.0 to support genomic and metagenomic analyses.

Indexed as

Genome, BacterialPhylogenyBacteriaGenome, MicrobialMetagenomeMetagenomics

Identifiers

PMID32427907
PMCPMC7237447
OpenAlexW3028280407

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.