ArticleNature communications2020
Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 266 papers.
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266 citing papers in PubMed.
- CRISPR/Cas9-mediated knockout ofaBIOTECH · 2026Article
- Assembly-graph-assisted haplotype-resolved scaffolding of polyploid genomes.Nature plants · 2026Article
- A miR65-MsbZIP11 post-transcriptional regulatory module coordinates saline-alkali stress tolerance in alfalfa (Medicago sativa L.).Plant cell reports · 2026Article
- From genome to gene module: decoding the BsAlfin2-BsTT2 regulatory network controlling low temperature-induced anthocyanin biosynthesis inHorticulture research · 2026Article
- Standardized microhaplotype databases and frameworks for assessing and mining crop genetic diversity.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Single-nucleus RNA-seq and ATAC-seq analyses provide molecular insights into cadmium-stress response in alfalfa roots.Horticulture research · 2026Article
- Polyploidization Redirects Carbon Flux to Diterpenoid Biosynthesis inPlants (Basel, Switzerland) · 2026Article
- Identification of race-specific QTL and candidate genes for Aphanomyces root rot resistance in alfalfa (Medicago sativa L.).G3 (Bethesda, Md.) · 2026Article
- Physiological and Transcriptomic Evaluation of Drought Tolerance in Alfalfa (Plants (Basel, Switzerland) · 2026Article
- Genome-Wide Identification of the PIN-LIKES (Current issues in molecular biology · 2026Article
- High-Contiguity Haplotype-Resolved Genome Assembly of the Hexaploid Actinidia valvata Rootstock Sheds Light on Waterlogging Resistance Gene.Plant biotechnology journal · 2026Article
- The MsZFP1-MsWRKY40-MsWRKY41 module efficiently regulates LHCII biogenesis via ABA-dependent manner in alfalfa.Science advances · 2026Article
- Genome-wide identification and gene expression analysis of Formin homology 2 (FH2) genes in peanut.BMC plant biology · 2026Article
- Article
- Overcoming breeding barriers with genome editing in autopolyploid crops.BMC plant biology · 2026Review
- Haplotype-resolved telomere-to-telomere assembly and haplotype-aware annotation pipeline enable high-quality reannotation of threeHorticulture research · 2026Article
- Developing doubled haploid technology for alfalfa breeding.Fundamental research · 2026Review
- Characterization of the BPC genes in alfalfa and functional verification of MsBPC10 in salt tolerance.BMC plant biology · 2026Article
- Breeding of a Multifoliolate Alfalfa Population Using CRISPR/Cas9-Generated Mutants and Evaluation of Agronomic Traits and Nutritive Value.Plants (Basel, Switzerland) · 2026Article
- Review
206 more citing papers are in PubMed but not listed here.
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24 authors.
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Abstract
Artificially improving traits of cultivated alfalfa (Medicago sativa L.), one of the most important forage crops, is challenging due to the lack of a reference genome and an efficient genome editing protocol, which mainly result from its autotetraploidy and self-incompatibility. Here, we generate an allele-aware chromosome-level genome assembly for the cultivated alfalfa consisting of 32 allelic chromosomes by integrating high-fidelity single-molecule sequencing and Hi-C data. We further establish an efficient CRISPR/Cas9-based genome editing protocol on the basis of this genome assembly and precisely introduce tetra-allelic mutations into null mutants that display obvious phenotype changes. The mutated alleles and phenotypes of null mutants can be stably inherited in generations in a transgene-free manner by cross pollination, which may help in bypassing the debate about transgenic plants. The presented genome and CRISPR/Cas9-based transgene-free genome editing protocol provide key foundations for accelerating research and molecular breeding of this important forage crop.
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