Evidence map›Paper›PMID 32427100›Full record

ArticleeLife2020

mRNA stem-loops can pause the ribosome by hindering A-site tRNA binding.

Chen Bao, Sarah Loerch, Clarence Ling, Andrei A Korostelev, Nikolaus Grigorieff, Dmitri N Ermolenko

Open access · goldAbstract read
In one paragraph

Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed
3.3field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed, 67 citations in OpenAlex.

  1. ZmDLR9 is required for lateral root development via pre-rRNA processing in maize.The Plant journal : for cell and molecular biology · 2026
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  4. The roles and synthesis of inorganic polyphosphate inbioRxiv : the preprint server for biology · 2026
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  11. RNA Structure Coordinates Translation Across the Meiotic Program.bioRxiv : the preprint server for biology · 2026
    Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Structural switching dynamically controls the doubly pseudoknotted Rous sarcoma virus-programmed ribosomal frameshifting element.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  17. Article
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  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Chen Bao *Department of Biochemistry and Biophysics at School of Medicine and Dentistry and Center for RNA Biology, University of Rochester, Rochester, United States.ORCID 0000-0002-9224-8083
Sarah Loerch *Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.ORCID 0000-0002-1731-516X
Clarence LingDepartment of Biochemistry and Biophysics at School of Medicine and Dentistry and Center for RNA Biology, University of Rochester, Rochester, United States.
Andrei A KorostelevDepartment of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, United States.ORCID 0000-0003-1588-717X
Nikolaus GrigorieffJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.ORCID 0000-0002-1506-909X
Dmitri N ErmolenkoDepartment of Biochemistry and Biophysics at School of Medicine and Dentistry and Center for RNA Biology, University of Rochester, Rochester, United States.ORCID 0000-0002-7554-5967
University of Rochester · USHoward Hughes Medical Institute · USUniversity of Massachusetts Chan Medical School · US

Funding

Virology/Immunology CoreP30AI078498 · NIAID · UNIVERSITY OF ROCHESTER · PI DEWHURST, STEPHEN · 2008 to 2018
$14.6M
Translational Control: Discovery and MechanismsR35GM127094 · NIGMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI Andrei Korostelev · 2018 to 2026
$5.5M
Structural Dynamics of TranslationR01GM099719 · NIGMS · UNIVERSITY OF ROCHESTER · PI ERMOLENKO, DMITRI · 2012 to 2020
$2.8M
NIAID NIH HHS P30 AI078498NIGMS NIH HHS R01 GM099719NIGMS NIH HHS R35 GM127094
6 · The paper itself

Abstract

Although the elongating ribosome is an efficient helicase, certain mRNA stem-loop structures are known to impede ribosome movement along mRNA and stimulate programmed ribosome frameshifting via mechanisms that are not well understood. Using biochemical and single-molecule Förster resonance energy transfer (smFRET) experiments, we studied how frameshift-inducing stem-loops from

Indexed as

Nucleic Acid ConformationRibosomesBacterial ProteinsCryoelectron MicroscopyDNA Polymerase IIIEscherichia coliFrameshifting, RibosomalFusion Proteins, gag-polGene Expression Regulation, BacterialGene Expression Regulation, ViralHIV-1RNA, BacterialRNA, MessengerRNA, TransferBacterial ProteinsDNA Polymerase IIIDnaX protein, BacteriaFusion Proteins, gag-polRNA, BacterialRNA, MessengerRNA, Transferchromosomescryo-EME. coligene expressionmolecular biophysicsmRNAribosomesmFRETstructural biologytranslation

Identifiers

PMID32427100
PMCPMC7282821
OpenAlexW3026435876

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.