ArticleHeliyon2020
Robust DNA protocols for tropical soils.
Article in Heliyon, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 34 citations in OpenAlex.
- Boosting tree growth in the Amazon rainforest using Amazonian Dark Earths.BMC ecology and evolution · 2026Article
- An effective DNA extraction protocol optimized for tropical swamp peat samples.Microbiology spectrum · 2026Article
- A catalog of metagenome-assembled genomes from Amazonian forest and pasture soils.Microbiology resource announcements · 2025Article
- Harnessing the synergy of Urochloa brizantha and Amazonian Dark Earth microbiomes for enhanced pasture recovery.BMC microbiology · 2025Article
- Bacterial genomes recovered from litter's metagenomes in Amazonian Dark Earths.Microbiology resource announcements · 2024Article
- Methane-cycling microbial communities from Amazon floodplains and upland forests respond differently to simulated climate change scenarios.Environmental microbiome · 2024Article
- Design and optimization of an improved qPCR assay for the detection of Histoplasma capsulatum.European journal of microbiology & immunology · 2023Article
- Insights into the Genomic Potential of aMicroorganisms · 2022Article
- Metagenomes from Eastern Brazilian Amazonian Floodplains in the Wet and Dry Seasons.Microbiology resource announcements · 2022Article
- Genome-resolved metagenomics reveals novel archaeal and bacterial genomes from Amazonian forest and pasture soils.Microbial genomics · 2022Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studies in the Amazon are being intensified to evaluate the alterations in the microbial communities of soils and sediments in the face of increasing deforestation and land-use changes in the region. However, since these environments present highly heterogeneous physicochemical properties, including contaminants that hinder nucleic acids isolation and downstream techniques, the development of best molecular practices is crucial. This work aimed to optimize standard protocols for DNA extraction and gene quantification by quantitative real-time PCR (qPCR) based on natural and anthropogenic soils and sediments (primary forest, pasture, Amazonian Dark Earth, and várzea, a seasonally flooded area) of the Eastern Amazon. Our modified extraction protocol increased the fluorometric DNA concentration by 48%, reaching twice the original amount for most of the pasture and várzea samples, and the 260/280 purity ratio by 15% to values between 1.8 to 2.0, considered ideal for DNA. The addition of bovine serum albumin in the qPCR reaction improved the quantification of the 16S rRNA genes of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.