ArticleWater research2020
SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area.
Article in Water research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 625 papers, 3 of them syntheses that pooled it.
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Who cites it
625 citing papers in PubMed, 3 syntheses or guidelines pooled it, 1,214 citations in OpenAlex.
- A Systematic Review of Methodological Approaches to SARS-CoV-2 Wastewater Surveillance.Viruses · 2026Pooled it
- Prevalence and Molecular Epidemiology of Human Coronaviruses in Africa Prior to the SARS-CoV-2 Outbreak: A Systematic Review.Viruses · 2023Pooled it
- Recent progress on wastewater-based epidemiology for COVID-19 surveillance: A systematic review of analytical procedures and epidemiological modeling.The Science of the total environment · 2023Pooled it
- Toward a quality-managed operational architecture for wastewater surveillance of zoonotic and emerging pathogens.Applied and environmental microbiology · 2026Review
- A 3-year wastewater-based epidemiology study of noroviruses in Senegal.Applied and environmental microbiology · 2026Article
- Statewide multi-year wastewater sequencing reveals dual origins of HIV-1 signal.Nature communications · 2026Article
- An integrated framework for early detection and transmissibility assessment of emerging variants in wastewater.International journal of hygiene and environmental health · 2026Article
- Evaluating Viral Pollution in Wastewater and Mediterranean Ecosystems.Food and environmental virology · 2026Article
- Designing effective SARS-CoV-2 wastewater surveillance system: lessons learnt from sustained monitoring in Delhi, India.BMC infectious diseases · 2026Article
- Remotely Controlled Continuous Surveillance of Viral RNA in Wastewater Using a LoRa Network.IEEE internet of things journal · 2026Article
- Environmental Surveillance of Norovirus RNA in Restaurant Settings: Cleaning Materials as Primary Viral Reservoirs.International journal of environmental research and public health · 2026Article
- Wastewater-based surveillance as a proactive public health tool: insights from SARS-CoV-2 monitoring in Kampala, Uganda (2023-2024).BMC public health · 2026Article
- SARS-CoV-2 wastewater genomic surveillance: approaches, challenges, and opportunities.Genome biology · 2026Review
- A One-Year Wastewater-Based Surveillance Study of the Main Human Respiratory Viruses in a Middle-Size Spanish City During the COVID-19 Pandemic Period.Microorganisms · 2026Article
- Methods for detecting highly pathogenic avian influenza H5N1 virus in dairy processing environments.Frontiers in microbiology · 2026Article
- Wastewater genomic surveillance identifies the emergence of the SARS-CoV-2 JN.1 lineage in urban settings of Gujarat.Frontiers in microbiology · 2026Article
- Review
- Incidence and temporal distribution of SARS-CoV-2 circulating in the municipal wastewaters of the Buffalo City region, Eastern Cape, South Africa.Global epidemiology · 2025Article
- SARS-CoV-2 Detection in International Travelers Through Wastewater-Based Epidemiology at the Kigali International Airport: Genomic Surveillance Study.JMIR public health and surveillance · 2025Article
- Genomic fragment detection and infectivity evaluation of rotaviruses isolated from wastewater used for irrigation in western Bogotá, D. C.Biomedica : revista del Instituto Nacional de Salud · 2025Article
565 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused more than 200,000 reported COVID-19 cases in Spain resulting in more than 20,800 deaths as of April 21, 2020. Faecal shedding of SARS-CoV-2 RNA from COVID-19 patients has extensively been reported. Therefore, we investigated the occurrence of SARS-CoV-2 RNA in six wastewater treatments plants (WWTPs) serving the major municipalities within the Region of Murcia (Spain), the area with the lowest COVID-19 prevalence within Iberian Peninsula. Firstly, an aluminum hydroxide adsorption-precipitation concentration method was validated using a porcine coronavirus (Porcine Epidemic Diarrhea Virus, PEDV) and mengovirus (MgV). The procedure resulted in average recoveries of 10 ± 3.5% and 10 ± 2.1% in influent water (n = 2) and 3.3 ± 1.6% and 6.2 ± 1.0% in effluent water (n = 2) samples for PEDV and MgV, respectively. Then, the method was used to monitor the occurrence of SARS-CoV-2 from March 12 to April 14, 2020 in influent, secondary and tertiary effluent water samples. By using the real-time RT-PCR (RT-qPCR) Diagnostic Panel validated by US CDC that targets three regions of the virus nucleocapsid (N) gene, we estimated quantification of SARS-CoV-2 RNA titers in untreated wastewater samples of 5.4 ± 0.2 log
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