ArticleNature chemical biology2020
Design of orthogonal regulatory systems for modulating gene expression in plants.
Article in Nature chemical biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 114 citations in OpenAlex.
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- ThePlants (Basel, Switzerland) · 2026Review
- A synthetic expression system for orthogonal gene expression in Nicotiana benthamiana.Plant molecular biology · 2026Article
- The Promise of Synthetic Biology for Redesigning Plant Architecture.International journal of molecular sciences · 2026Review
- Remote Sensing of Endogenous Pigmentation by Inducible Synthetic Circuits in Grasses.Plant biotechnology journal · 2026Article
- Causes and consequences of experimental variation in Nicotiana benthamiana transient expression.Nature communications · 2026Article
- A toolkit for programmable transcriptional engineering across eukaryotic kingdoms.bioRxiv : the preprint server for biology · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Article
- Engineering glowing plants: recent progress and future directions for application-oriented design.Frontiers in bioengineering and biotechnology · 2026Review
- Binary vector copy number engineering improves Agrobacterium-mediated transformation.Nature biotechnology · 2025Article
- Identification and application of bioparts for plant synthetic biology.Molecules and cells · 2025Review
- Programmable genome engineering and gene modifications for plant biodesign.Plant communications · 2025Review
- Toward a monocot SynBio toolkit: assessing regulatory element performance and eudicot compatibility.Plant cell reports · 2025Article
- Through the lens of bioenergy crops: advances, bottlenecks, and promises of plant engineering.The Plant journal : for cell and molecular biology · 2025Review
- Harnessing promoter elements to enhance gene editing in plants: perspectives and advances.Plant biotechnology journal · 2025Review
- CRISPRi-based circuits to control gene expression in plants.Nature biotechnology · 2025Article
- Navigating the challenges of engineering composite specialized metabolite pathways in plants.The Plant journal : for cell and molecular biology · 2025Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Agricultural biotechnology strategies often require the precise regulation of multiple genes to effectively modify complex plant traits. However, most efforts are hindered by a lack of characterized tools that allow for reliable and targeted expression of transgenes. We have successfully engineered a library of synthetic transcriptional regulators that modulate expression strength in planta. By leveraging orthogonal regulatory systems from Saccharomyces spp., we have developed a strategy for the design of synthetic activators, synthetic repressors, and synthetic promoters and have validated their use in Nicotiana benthamiana and Arabidopsis thaliana. This characterization of contributing genetic elements that dictate gene expression represents a foundation for the rational design of refined synthetic regulators. Our findings demonstrate that these tools provide variation in transcriptional output while enabling the concerted expression of multiple genes in a tissue-specific and environmentally responsive manner, providing a basis for generating complex genetic circuits that process endogenous and environmental stimuli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.