ArticleScientific reports2020
Combined Atomic Force Microscope and Volumetric Light Sheet System for Correlative Force and Fluorescence Mechanobiology Studies.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Using tunable hydrogel microparticles to measure cellular forces.Nature protocols · 2026Review
- Nanoscale Analysis beyond Imaging by Atomic Force Microscopy: Molecular Perspectives on Oncology and Neurodegeneration.Small science · 2025Review
- Actin from within - how nuclear myosins and actin regulate nuclear architecture and mechanics.Journal of cell science · 2025Review
- A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.Journal of applied physics · 2024Article
- Microscopy methods to visualize nuclear organization in biomechanical studies.Current opinion in biomedical engineering · 2024Article
- Mechanisms of frustrated phagocytic spreading of human neutrophils on antibody-coated surfaces.Biophysical journal · 2022Article
- Targeting cell-matrix interface mechanobiology by integrating AFM with fluorescence microscopy.Progress in biophysics and molecular biology · 2022Review
- Engineering physical microenvironments to study innate immune cell biophysics.APL bioengineering · 2022Review
- Building the phagocytic cup on an actin scaffold.Current opinion in cell biology · 2022Review
- Integrative experimental/computational approach establishes active cellular protrusion as the primary driving force of phagocytic spreading by immune cells.PLoS computational biology · 2022Article
- Combining multiple fluorescence imaging techniques in biology: when one microscope is not enough.Molecular biology of the cell · 2022Article
- Forced into shape: Mechanical forces in Drosophila development and homeostasis.Seminars in cell & developmental biology · 2021Review
- A survey of physical methods for studying nuclear mechanics and mechanobiology.APL bioengineering · 2021Review
- Using blinking optical tweezers to study cell rheology during initial cell-particle contact.Biophysical journal · 2021Article
- Review
- Regulation of Nuclear Mechanics and the Impact on DNA Damage.International journal of molecular sciences · 2021Review
- Nuclear Deformation Causes DNA Damage by Increasing Replication Stress.Current biology : CB · 2021Article
- Visualizing the Invisible: Advanced Optical Microscopy as a Tool to Measure Biomechanical Forces.Frontiers in cell and developmental biology · 2021Review
- AFM-Based Correlative Microscopy Illuminates Human Pathogens.Frontiers in cellular and infection microbiology · 2021Review
- What Is the Right Mechanical Readout for Understanding the Mechanobiology of the Immune Response?Frontiers in cell and developmental biology · 2021Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
The central goals of mechanobiology are to understand how cells generate force and how they respond to environmental mechanical stimuli. A full picture of these processes requires high-resolution, volumetric imaging with time-correlated force measurements. Here we present an instrument that combines an open-top, single-objective light sheet fluorescence microscope with an atomic force microscope (AFM), providing simultaneous volumetric imaging with high spatiotemporal resolution and high dynamic range force capability (10 pN - 100 nN). With this system we have captured lysosome trafficking, vimentin nuclear caging, and actin dynamics on the order of one second per single-cell volume. To showcase the unique advantages of combining Line Bessel light sheet imaging with AFM, we measured the forces exerted by a macrophage during FcɣR-mediated phagocytosis while performing both sequential two-color, fixed plane and volumetric imaging of F-actin. This unique instrument allows for a myriad of novel studies investigating the coupling of cellular dynamics and mechanical forces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.