ArticleNature communications2020
Freeze-frame imaging of synaptic activity using SynTagMA.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 54 citations in OpenAlex.
- CaMPARI2 enables stimulus-locked whole-brain activity mapping at cellular resolution in unrestrained larval zebrafish.Frontiers in molecular neuroscience · 2026Article
- CaMPARI2 Enables Stimulus-Locked Whole-Brain Activity Mapping at Cellular Resolution in Unrestrained Larval Zebrafish.bioRxiv : the preprint server for biology · 2025Article
- Single-cell synaptome mapping of endogenous protein subpopulations in mammalian brain.Nature communications · 2025Article
- CRISPRi-based screen of autism spectrum disorder risk genes in microglia uncovers roles of ADNP in microglia endocytosis and synaptic pruning.Molecular psychiatry · 2025Article
- Review
- Distinct spatial distribution of potentiated dendritic spines in encoding- and recall-activated hippocampal neurons.Frontiers in molecular neuroscience · 2025Article
- CRISPRi-based screen of Autism Spectrum Disorder risk genes in microglia uncovers roles ofbioRxiv : the preprint server for biology · 2024Article
- Advances in the labelling and selective manipulation of synapses.Nature reviews. Neuroscience · 2024Review
- Fiber-basedNeurophotonics · 2024Article
- Molecular tools to capture active neural circuits.Frontiers in neural circuits · 2024Review
- Approaches and considerations of studying neuronal ensembles: a brief review.Frontiers in cellular neuroscience · 2023Review
- Spike-timing-dependent plasticity rewards synchrony rather than causality.Cerebral cortex (New York, N.Y. : 1991) · 2022Article
- Exploiting the molecular diversity of the synapse to investigate neuronal communication: A guide through the current toolkit.The European journal of neuroscience · 2022Review
- BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons.Nature communications · 2021Article
- FluoSTEPs: Fluorescent biosensors for monitoring compartmentalized signaling within endogenous microdomains.Science advances · 2021Article
- Freeze-Frame Imaging of Dendritic Calcium Signals With TubuTag.Frontiers in molecular neuroscience · 2021Article
- Towards a Comprehensive Optical Connectome at Single Synapse ResolutionFrontiers in synaptic neuroscience · 2021Review
- RNA NTrends in neurosciences · 2020Review
- Article
- Neuronal Activity at Synapse Resolution: Reporters and Effectors for Synaptic Neuroscience.Frontiers in molecular neuroscience · 2020Review
Corrections and comments
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Authors and funding
15 authors at 4 institutions in 3 countries.
Funding
Abstract
Information within the brain travels from neuron to neuron across billions of synapses. At any given moment, only a small subset of neurons and synapses are active, but finding the active synapses in brain tissue has been a technical challenge. Here we introduce SynTagMA to tag active synapses in a user-defined time window. Upon 395-405 nm illumination, this genetically encoded marker of activity converts from green to red fluorescence if, and only if, it is bound to calcium. Targeted to presynaptic terminals, preSynTagMA allows discrimination between active and silent axons. Targeted to excitatory postsynapses, postSynTagMA creates a snapshot of synapses active just before photoconversion. To analyze large datasets, we show how to identify and track the fluorescence of thousands of individual synapses in an automated fashion. Together, these tools provide an efficient method for repeatedly mapping active neurons and synapses in cell culture, slice preparations, and in vivo during behavior.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.