ArticleAnaerobe2020
Clostridioides difficile proline fermentation in response to commensal clostridia.
Article in Anaerobe, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- Mechanisms by which proline reductase ofmBio · 2026Article
- Ecological and dietary strategies to constrain Clostridioides difficile.Cell host & microbe · 2026Review
- Design and application of synthetic human gut microbial communities.Gut microbes · 2025Review
- Unravelling three-way interactions betweenJournal of medical microbiology · 2025Review
- Nutrient acquisition strategies by gut microbes.Cell host & microbe · 2024Review
- Review
- d-Proline Reductase Underlies Proline-Dependent Growth of Clostridioides difficile.Journal of bacteriology · 2022Article
- The Stickland Reaction PrecursormSphere · 2022Article
- Effect of diet on pathogen performance in the microbiome.Microbiome research reports · 2022Review
- Article
- Contribution of Inhibitory Metabolites and Competition for Nutrients to Colonization Resistance againstMicroorganisms · 2021Review
- Microbial adaptation to the healthy and inflamed gut environments.Gut microbes · 2020Review
- Mouse models for bacterial enteropathogen infections: insights into the role of colonization resistance.Gut microbesArticle
- Gut associated metabolites and their roles inGut microbesArticle
- The development of live biotherapeutics againstGut microbesArticle
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
Clostridioides difficile colonizes the intestines of susceptible individuals and releases toxins that mediate disease. To replicate and expand in the intestines, C. difficile ferments proline, and this activity is influenced by the availability of proline and trace nutrients. C. difficile must also compete with the commensal microbiota for these limited nutrients. The specific microbes present in the intestines that may shape the ability of C. difficile to benefit from proline fermentation are unknown. In this study we developed a panel of commensal Clostridia to test the hypothesis that the microbiota influences C. difficile growth through proline fermentation. The experimental panel of Clostridia was composed of murine and human isolates that ranged in their capacity to ferment proline in different media. Competition between wild type C. difficile and a mutant strain unable to ferment proline (prdB:CT) in the presence of these Clostridia revealed that bacteria closely related to Paraclostridium benzoelyticum and Paeniclostridium spp. decreased the benefit to C. difficile provided by proline fermentation. Conversely, Clostridium xylanolyticum drove C. difficile towards an increased reliance on proline fermentation for growth. Overall, the ability of C. difficile to benefit from proline fermentation is contextual and in part dependent on the microbiota.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.