ArticleMolecular plant pathology2020
Pm21 CC domain activity modulated by intramolecular interactions is implicated in cell death and disease resistance.
Article in Molecular plant pathology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 30 citations in OpenAlex.
- An Auto-Activated NLR-Protein OsRGA3Plant biotechnology journal · 2026Article
- Functional Dissection of a Wheat NLR Protein Reveals a Minimal Active Region and Key Regulatory Sites for Immune Signalling.Molecular plant pathology · 2026Article
- An NLR pair in the Pm68 locus confers powdery mildew resistance in durum and common wheat.Nature communications · 2025Article
- Genome-Wide Characterization and Expression Pattern Analysis Insights into PlantInternational journal of molecular sciences · 2025Article
- The wheat NLR pair RXL/Pm5e confers resistance to powdery mildew.Plant biotechnology journal · 2025Article
- An Aegilops longissima NLR protein with integrated CC-BED module mediates resistance to wheat powdery mildew.Nature communications · 2024Article
- A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew.Nature communications · 2024Article
- Activation and Autoinhibition Mechanisms of NLR Immune Receptor Pi36 in Rice.International journal of molecular sciences · 2024Article
- Two functional CC-NBS-LRR proteins from rye chromosome 6RS confer differential age-related powdery mildew resistance to wheat.Plant biotechnology journal · 2024Article
- Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides.Nature communications · 2023Article
- Orthologous genes Pm12 and Pm21 from two wild relatives of wheat show evolutionary conservation but divergent powdery mildew resistance.Plant communications · 2023Article
- Genetic mapping of powdery mildew resistance genes in wheat landrace Guizi 1 via genotyping by sequencing.Molecular biology reports · 2022Article
- Large-scale mutational analysis of wheat powdery mildew resistance geneFrontiers in plant science · 2022Article
- PM2b, a CC-NBS-LRR protein, interacts with TaWRKY76-D to regulate powdery mildew resistance in common wheat.Frontiers in plant science · 2022Article
- Pm21 CC domain activity modulated by intramolecular interactions is implicated in cell death and disease resistance.Molecular plant pathology · 2020Article
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10 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Nucleotide-binding (NB) leucine-rich repeat (LRR) receptors (NLRs) provide resistance against several plant pathogens. We previously cloned the wheat powdery mildew resistance gene Pm21, which encodes a coiled-coil (CC) NLR that confers broad-spectrum resistance against Blumeria graminis f. sp. tritici. Here, we report comprehensive biochemical and functional analyses of Pm21 CC domain in Nicotiana benthamiana. Transient overexpression assay suggested that only the extended CC (eCC, amino acid residues 1-159) domain has cell-death-inducing activity, whereas the CC-containing truncations, including CC-NB and CC-NB-LRR, do not induce cell-death responses. Coimmunoprecipitation (Co-IP) assay showed that the eCC domain self-associates and interacts with the NB and LRR domains in planta. These results imply that the activity of the eCC domain is inhibited by the intramolecular interactions of different domains in the absence of pathogens. We found that the LRR domain plays a crucial role in D491V-mediated full-length (FL) Pm21 autoactivation. Some mutations in the CC domain leading to the loss of Pm21 resistance to powdery mildew impaired the CC activity of cell-death induction. Two mutations (R73Q and E80K) interfered with D491V-mediated Pm21 autoactivation without affecting the cell-death-inducing activity of the eCC domain. Notably, some susceptible mutants harbouring mutations in the CC domain still exhibited cell-death-inducing activity. Taken together, these results implicate the CC domain of Pm21 in cell-death signalling and disease-resistance signalling, which are potentially independent of each other.
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