Evidence map›Paper›PMID 32420381›Full record

ArticleBioMed research international2020

A Novel Strategy Facilitates Reference Gene Selection by RT-qPCR Analysis in Kidney Yang Deficiency Syndrome Mice Infected with the Influenza A (H1N1) Virus.

Yepei Fu, Jia Yang, Shanshan Fan, Shaozhe Zhao, Syed Muhammad Ali Shah, Muhammad Akram, Rong Rong, Yong Yang

Open access · hybridAbstract read
In one paragraph

Article in BioMed research international, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Yepei FuShandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, China.
Jia YangShandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, China.
Shanshan FanShandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, China.
Shaozhe ZhaoShandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, China.
Syed Muhammad Ali ShahDepartment of Eastern Medicine, Directorate of Medical Sciences, Government College University, Faisalabad 38000, Pakistan.
Muhammad AkramDepartment of Eastern Medicine, Directorate of Medical Sciences, Government College University, Faisalabad 38000, Pakistan.ORCID https://orcid.org/0000-0002-7457-8572
Rong RongShandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, China.ORCID https://orcid.org/0000-0001-6261-9202
Yong YangShandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, China.ORCID https://orcid.org/0000-0003-3298-1440
Shandong University of Traditional Chinese Medicine · CNGovernment College University, Faisalabad · PK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In reverse transcription-quantitative polymerase chain reaction (RT-qPCR) studies, endogenous reference genes are routinely used to normalize the expression of target gene studies. In order to precisely evaluate the relative expression of genes in the cells of mice suffering from Kidney Yang Deficiency Syndrome (KYDS) in response to influenza A virus (IAV) H1N1 using RT-qPCR, it is crucial to identify reliable reference genes. In the present study, 15 candidate reference genes (Actb,

Indexed as

AlgorithmsInfluenza A Virus, H1N1 SubtypeKidney DiseasesOrthomyxoviridae InfectionsYang DeficiencyAnimalsGene Expression ProfilingMaleMiceMice, Inbred BALB CReal-Time Polymerase Chain ReactionReference StandardsReverse Transcriptase Polymerase Chain Reaction

Identifiers

PMID32420381
PMCPMC7201461
OpenAlexW3017680590

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.