ArticleTranslational andrology and urology2020
Analysis of bacterial community using pyrosequencing in semen from patients with chronic pelvic pain syndrome: a pilot study.
Article in Translational andrology and urology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed.
- Commentary on "Global impact of pelvic inflammatory diseases attributable to sexually transmitted infections in women of childbearing age: a comprehensive analysis based on GBD 2021 data and bibliometric analysis" (Letter).International journal of surgery (London, England) · 2026Article
- The role of microbiota in the chronic prostatitis/chronic pelvis pain syndrome: a review.Frontiers in microbiology · 2025Review
- Research progress on the relationship between chronic prostatitis/chronic pelvic pain syndrome and the microbiota of the reproductive system.Frontiers in cellular and infection microbiology · 2024Review
- Article
- Microbiology and immune mechanisms associated with male infertility.Frontiers in immunology · 2023Review
- Possibilities and limitations of using low biomass samples for urologic disease and microbiome research.Prostate international · 2022Review
- Prostate diseases and microbiome in the prostate, gut, and urine.Prostate international · 2022Review
- [The role of the microbiome in urology].Der Urologe. Ausg. A · 2020Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough empirical antibacterial treatments are currently recommended for inflammatory chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS), physicians cannot verify infections in most cases. Therefore, in this study, the microbiota of semen was investigated via pyrosequencing to obtain evidence underlying infectious disease.
methodsPatients diagnosed with CP/CPPS (n=17) and healthy volunteers (n=4) participated in the study. Whole DNA was purified from the participants' semen. The DNA was amplified by polymerase chain reaction (PCR) using universal bacterial primers. All semen samples were also cultured using conventional methods. Pyrosequencing analysis of the PCR-amplified DNA was performed.
resultsNone of the semen samples showed colony formation in conventional bacterial cultures. However, pyrosequencing revealed multiple bacterial genera in all samples, including an abundance of fastidious bacteria.
conclusionsThe identification of various dominant species in the CP/CPPS group other than those reported in previous studies might be helpful for future etiological analysis of CP/CPPS.
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