ReviewMetabolomics : Official journal of the Metabolomic Society2020
A review of strategies for untargeted urinary metabolomic analysis using gas chromatography-mass spectrometry.
Review in Metabolomics : Official journal of the Metabolomic Society, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 75 citations in OpenAlex.
- Separative Techniques Coupled to Mass Spectrometry for Metabolomic Analysis.Advances in experimental medicine and biology · 2026Review
- Integrating untargeted volatile metabolomics and molecular evidence supporting chemotaxonomy in Kaempferia species for more effective identification.Scientific reports · 2025Article
- Mass spectrometry-based metabolomic as a powerful tool to unravel the component and mechanism in TCM.Chinese medicine · 2025Review
- A Novel Fiber-Optical Fabry-Perot Microtip Sensor for 2-Propanol.Sensors (Basel, Switzerland) · 2025Article
- From Complexity to Clarity: Expanding Metabolome Coverage With Innovative Analytical Strategies.Journal of separation science · 2025Review
- Urine organic acid metabolomic profiling by gas chromatography mass spectrometry: Assessment of solvent extract evaporation parameters on the recovery of key diagnostic metabolites.Clinica chimica acta; international journal of clinical chemistry · 2025Article
- Innovative molecular networking analysis of steroids and characterisation of the urinary steroidome.Scientific data · 2024Article
- Urinary Metabolomic Differentiation of Infants Fed on Human Breastmilk and Formulated Milk.Metabolites · 2024Article
- Determination of pesticide residues in urine by chromatography-mass spectrometry: methods and applications.Frontiers in public health · 2024Review
- Carotenoids in Health as Studied by Omics-Related Endpoints.Advances in nutrition (Bethesda, Md.) · 2023Review
- Optimising a urinary extraction method for non-targeted GC-MS metabolomics.Scientific reports · 2023Article
- Role of untargeted omics biomarkers of exposure and effect for tobacco research.Addiction neuroscience · 2023Article
- Metabolomics Analysis of DRG and Serum in the CCI Model of Mice.Brain sciences · 2023Article
- Denoising Autoencoder Normalization for Large-Scale Untargeted Metabolomics by Gas Chromatography-Mass Spectrometry.Metabolites · 2023Article
- Compound-Specific 1DACS omega · 2023Article
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- Towards inferring absolute concentrations from relative abundance in time-course GC-MS metabolomics data.Molecular omics · 2023Article
- Metabolic profiling reveals metabolic features of consolidation therapy in pediatric acute lymphoblastic leukemia.Cancer & metabolism · 2023Article
- Current State and Future Perspectives on Personalized Metabolomics.Metabolites · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHuman urine gives evidence of the metabolism in the body and contains different metabolites at various concentrations. A number of analytical techniques including mass spectrometry (MS) and nuclear magnetic resonance (NMR) have been used to obtain metabolites levels in urine samples. However, gas chromatography-mass spectrometry (GC-MS) is one of the most widely used techniques for urinary metabolomics studies due to its higher sensitivity, resolution, reproducibility, reliability, relatively low cost and ease of operation compared to liquid chromatography-mass spectrometry and NMR. AIM OF REVIEW: This review looks at various aspects of urine preparation prior to analysis by GC-MS including sample storage, urease pretreatment, derivatization, use of internal standard and quality control samples for data correction. In addition, most common types of inlet liners, ionization techniques and columns are discussed and a summary of mass analyzers are also highlighted. Lastly, the role of retention index in metabolite identification and data normalization methods are presented. KEY SCIENTIFIC CONCEPTS OF REVIEW: The purpose of this review is summarizing methods of sample storage, pretreatment, and GC-MS analysis that are mostly used in urine metabolomics studies. Specific emphasis is given to the critical steps within the GC-MS urine metabolomics that those new to this field need to be aware of and the remaining challenges that require further attention and studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.