ArticleBiochimica et biophysica acta. Biomembranes2020
Characterization of lipid composition and diffusivity in OLA generated vesicles.
Article in Biochimica et biophysica acta. Biomembranes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 36 citations in OpenAlex.
- Lipid remodeling enables adaptation to chronic hyperosmotic stress.The Journal of cell biology · 2026Article
- Systematic investigation of double emulsion dewetting dynamics for the robust production of giant unilamellar vesicles.Lab on a chip · 2026Article
- Mechanical performance of hybrid polymer-lipid vesicles with leaflet asymmetry engineered using microfluidics.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Enhanced Diffusion of Single, Lipid-Tethered Enzymes.Nano letters · 2026Article
- Measuring Vesicle Loading with Holographic Microscopy and Bulk Light Scattering.ACS physical chemistry Au · 2024Article
- Polysaccharide functionalization reduces lipid vesicle stiffness.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Protein-Rich Rafts in Hybrid Polymer/Lipid Giant Unilamellar Vesicles.Biomacromolecules · 2024Article
- Formation of Giant Unilamellar Vesicles Assisted by Fluorinated Nanoparticles.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Measuring Thousands of Single-Vesicle Leakage Events Reveals the Mode of Action of Antimicrobial Peptides.Analytical chemistry · 2022Article
- A Surfactant Enables Efficient Membrane Spanning by Non-Aggregating DNA-Based Ion Channels.Molecules (Basel, Switzerland) · 2022Article
- A Microfluidic Platform for Sequential Assembly and Separation of Synthetic Cell Models.ACS synthetic biology · 2021Article
- Optimized cDICE for Efficient Reconstitution of Biological Systems in Giant Unilamellar Vesicles.ACS synthetic biology · 2021Article
- Standardizing characterization of membrane active peptides with microfluidics.Biomicrofluidics · 2021Article
- Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics.Communications chemistry · 2021Article
- Proton gradients from light-harvesting E. coli control DNA assemblies for synthetic cells.Nature communications · 2021Article
- Switching Cytolytic Nanopores into Antimicrobial Fractal Ruptures by a Single Side Chain Mutation.ACS nano · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Giant Unilamellar Vesicles (GUVs) are a versatile tool in many branches of science, including biophysics and synthetic biology. Octanol-Assisted Liposome Assembly (OLA), a recently developed microfluidic technique enables the production and testing of GUVs within a single device under highly controlled experimental conditions. It is therefore gaining significant interest as a platform for use in drug discovery, the production of artificial cells and more generally for controlled studies of the properties of lipid membranes. In this work, we expand the capabilities of the OLA technique by forming GUVs of tunable binary lipid mixtures of DOPC, DOPG and DOPE. Using fluorescence recovery after photobleaching we investigated the lateral diffusion coefficients of lipids in OLA liposomes and found the expected values in the range of 1 μm
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.