Evidence map›Paper›PMID 32416191›Full record

ArticleBiochimica et biophysica acta. Biomembranes2020

The SecA ATPase motor protein binds to Escherichia coli liposomes only as monomers.

Guillaume Roussel, Stephen H White

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochimica et biophysica acta. Biomembranes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. The Dynamic SecYEG Translocon.Frontiers in molecular biosciences · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Guillaume RousselDepartment of Physiology and Biophysics, School of Medicine, University of California, Irvine, Irvine, CA 92697, United States of America.
Stephen H WhiteDepartment of Physiology and Biophysics, School of Medicine, University of California, Irvine, Irvine, CA 92697, United States of America. Electronic address: stephen.white@uci.edu.
University of California, Irvine · US

Funding

Membrane Protein Folding and AssemblyR01GM074637 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI WHITE, STEPHEN H. · 2006 to 2020
$4.5M
Membrane Protein Folding and AssemblyR35GM139652 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI WHITE, STEPHEN H. · 2021 to 2025
$2.0M
NIGMS NIH HHS R01 GM074637NIGMS NIH HHS R35 GM139652
6 · The paper itself

Abstract

The essential SecA motor ATPase acts in concert with the SecYEG translocon to secrete proteins into the periplasmic space of Escherichia coli. In aqueous solutions, SecA exists largely as dimers, but the oligomeric state on membranes is less certain. Crystallographic studies have suggested several possible solution dimeric states, but its oligomeric state when bound to membranes directly or indirectly via the translocon is controversial. We have shown using disulfide crosslinking that the principal solution dimer, corresponding to a crystallographic dimer (PDB 1M6N), binds only weakly to large unilamellar vesicles (LUV) formed from E. coli lipids. We report here that other soluble crosslinked crystallographic dimers also bind weakly, if at all, to LUV. Furthermore, using a simple glutaraldehyde crosslinking scheme, we show that SecA is always monomeric when bound to LUV formed from E. coli lipids.

Indexed as

Cell MembraneEscherichia coliEscherichia coli ProteinsLiposomesProtein BindingProtein MultimerizationSecA ProteinsEscherichia coli ProteinsLiposomesSecA protein, E coliSecA ProteinsDisulfide crosslinkingGlutaraldehyde crosslinkingLarge unilamellar vesicles (LUV)Protein partitioningProtein secretion

Identifiers

PMID32416191
PMCPMC7316483
OpenAlexW3027635449

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.