ArticleJournal of animal science2020
Ruminal microbes of adult sheep do not degrade extracellular l-citrulline.
Article in Journal of animal science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Effects of Duodenal Infusion of L-Citrulline on Plasma Metabolism, Fecal Microbiota Structure, and Reproductive Hormones in Ewes.Life (Basel, Switzerland) · 2026Article
- Dietary L-Citrulline Supplementation Promotes Rumen Development and Modulates the Microbiota-Metabolome Axis in Suckling Hu Lambs.Animals : an open access journal from MDPI · 2026Article
- Effects of L-Citrulline Supplementation on Rumen Microbiota and Reproductive Performance of Ewes.Life (Basel, Switzerland) · 2026Article
- Maternal dietary citrulline supplementation increases fetal growth and programs pancreatic development in the lambs.Amino acids · 2026Article
- Use of melatonin plus citrulline or arginine to mitigate ergot alkaloid-induced vasoconstriction in sheep.Animal feed science and technology · 2025Article
- Dietary Supplementation with L-Citrulline Between Days 1 and 60 of Gestation Enhances Embryonic Survival in Lactating Beef Cows.Animals : an open access journal from MDPI · 2025Article
- Dietary citrulline supplementation enhances milk production in lactating dairy goats.Journal of animal science and biotechnology · 2025Article
- Impact of dietary supplementation of L-citrulline to meat goats during gestation on reproductive performance.Journal of animal science and biotechnology · 2025Article
- Effect of supplementation withFrontiers in microbiology · 2025Article
- The Effects ofAnimals : an open access journal from MDPI · 2024Article
- Metabolism and Nutrition of L-Glutamate and L-Glutamine in Ruminants.Animals : an open access journal from MDPI · 2024Review
- Study on the correlation of supplementation withFrontiers in microbiology · 2024Article
- Metabolomic and Transcriptomic Changes Underlying the Effects ofAnimals : an open access journal from MDPI · 2023Article
- Effects of mid-gestational l-citrulline supplementation to twin-bearing ewes on umbilical blood flow, placental development, and lamb production traits.Translational animal science · 2021Article
- Amino Acid Nutrition and Reproductive Performance in Ruminants.Advances in experimental medicine and biology · 2021Article
- Amino Acids in the Nutrition and Production of Sheep and Goats.Advances in experimental medicine and biology · 2021Review
- Effect of supplementation of unprotected or protected arginine to prolific ewes on maternal amino acids profile, lamb survival at birth, and pre- and post-weaning lamb growth.Journal of animal science · 2020Article
- Evaluation of oral citrulline administration as a mitigation strategy for fescue toxicosis in sheep.Translational animal science · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study determined whether extracellular citrulline is degraded by ruminal bacteria of sheep. In the first experiment, whole rumen fluid (3 mL) from six adult Suffolk sheep was incubated at 37 °C with 5 mM l-glutamine (Gln), l-glutamate (Glu), l-arginine (Arg), or l-citrulline (Cit) for 0, 0.5, 1, and 2 h or with 0, 0.5, 2, or 5 mM Gln, Glu, Arg, or Cit for 2 h. An aliquot (50 µL) of the incubation solution was collected at the predetermined time points for amino acids (AA) analyses. Results showed extensive hydrolysis of Gln into Glu and ammonia, of Arg into l-ornithine and l-proline, but little or no degradation of extracellular Cit or Glu by ruminal microbes. In the second experiment, six adult Suffolk sheep were individually fed each of three separate supplements (8 g Gln , Cit, or urea) on three separate days along with regular feed (800 g/animal). Blood (2 mL) was sampled from the jugular vein prior to feeding (time 0) and at 0.5, 1, 2, and 4 h after consuming the supplement. Plasma was analyzed for AA, glucose, ammonia, and urea. The concentrations of Cit in the plasma of sheep consuming this AA increased (P < 0.001) by 117% at 4 h and those of Arg increased by 23% at 4 h, compared with the baseline values. Urea or Gln feeding did not affect (P > 0.05) the concentrations of Cit or Arg in plasma. These results indicate that Cit is not metabolized by ruminal microbes of sheep and is, therefore, absorbed as such by the small intestine and used for the synthesis of Arg by extrahepatic tissues.
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