ArticleAMB Express2020
Characterization and antimicrobial activity of lectins purified from three Egyptian leguminous seeds.
Article in AMB Express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 42 citations in OpenAlex.
- Plant antimicrobials: extraction, characterization and activity against foodborne microorganisms.Folia microbiologica · 2026Review
- Purification, Characterization and Bioactivity of a New Homodimeric Lectin FromPlant-environment interactions (Hoboken, N.J.) · 2025Article
- Antibacterial and anticancer activities of three novel lectin-conjugated chitosan nanoparticles.Applied microbiology and biotechnology · 2024Article
- Bacterial Degradation of Antinutrients in Foods: The Genomic Insight.Foods (Basel, Switzerland) · 2024Review
- Applications of tandem mass spectrometry (MS/MS) in antimicrobial peptides field: Current state and new applications.Heliyon · 2024Review
- Health-promoting benefits of lentils: Anti-inflammatory and anti-microbial effects.Current research in physiology · 2024Review
- A Comprehensive Review of Pea (Foods (Basel, Switzerland) · 2023Review
- Antimicrobial activities and phylogenetic study ofBiotechnologia · 2023Article
- A review on anti-nutritional factors: unraveling the natural gateways to human health.Frontiers in nutrition · 2023Review
- Partial Characterization of Lectins Purified from the Surco and Vara (Furrow and Rod) Varieties of BlackMolecules (Basel, Switzerland) · 2022Article
- Isolation, purification and characterization of the antibacterial, antihypertensive and antioxidative properties of the bioactive peptides in the purified and proteolyzed major storage protein of pigeon pea (Food chemistry. Molecular sciences · 2022Article
- Review
- Widening the Perspectives for Legume Consumption: The Case of Bioactive Non-nutrients.Frontiers in plant science · 2022Article
- Genetic manipulation of anti-nutritional factors in major crops for a sustainable diet in future.Frontiers in plant science · 2022Review
- Research advances and prospects of legume lectins.Journal of biosciences · 2021Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lectins are carbohydrate-binding proteins that play vital roles in many biological processes. In this study, lectins from three Egyptian cultivars (fava bean, lentil, and pea) were isolated by precipitation with different concentrations of ammonium sulfate. The purification process was performed by affinity chromatography using mannose agarose. The highest concentration of purified lectins (1.48 mg/g) was recorded in pea at 90% saturation. SDS-PAGE of the purified lectins revealed bands of low molecular weights (14 to 18 kDa). The complete amino acid sequences of purified lectins were assessed using mass spectrometry (MS), which indicated the presence of the peptides favin, p54, and psl in fava bean, lentil, and pea, respectively. The lectins showed antimicrobial activity. The highest inhibition zone (35 mm) was measured with lectin purified from lentil against Staphylococcus aureus ATCC 6538, followed by pea lectin (33.4 mm) against Pseudomonas aeruginosa ATCC 10145. To the best of our knowledge, the legume lectins in this study are the first lectins to exhibit antifungal activity against Candida albicans, with the maximum inhibition zone (25.1 mm) observed with purified lectins of fava bean. Additionally, the first scanning electron microscope (SEM) images showing agglutination and clumping of microbial cells exposed to tested lectins are provided. These findings proved that Egyptian legume lectins are distinct from other lectins reported in previous studies and demonstrated their potential as antimicrobial agents against human pathogenic microorganisms.
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