ArticleScientific reports2020
Real-time multispectral fluorescence lifetime imaging using Single Photon Avalanche Diode arrays.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The trial behind it
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Who cites it
18 citing papers in PubMed, 58 citations in OpenAlex.
- Spatio-temporally modulated fluorescence imager for intraoperative nerve identification.Journal of biomedical optics · 2026Article
- Segmentation-guided photon pooling enables robust single-cell analysis and fast fluorescence lifetime imaging microscopy.Journal of biomedical optics · 2026Article
- Fluorescence Lifetime Imaging Techniques-A Review on Principles, Applications and Clinical Relevance.Journal of biophotonics · 2025Review
- Segmentation-guided photon pooling enables robust single cell analysis and fast fluorescence lifetime imaging microscopy.bioRxiv : the preprint server for biology · 2025Article
- High-throughput hyperspectral fluorescence imaging using a high-speed silicon photomultiplier array.Biomedical optics express · 2025Article
- Multispectral label-freeJournal of biomedical optics · 2024Article
- Dual excitation spectral autofluorescence lifetime and reflectance imaging for fast macroscopic characterization of tissues.Biomedical optics express · 2024Article
- Pulse-sampling fluorescence lifetime imaging: evaluation of photon economy.Optics letters · 2023Article
- A Guide to Perform 3D Histology of Biological Tissues with Fluorescence Microscopy.International journal of molecular sciences · 2023Review
- Review
- Automated Phasor Segmentation of Fluorescence Lifetime Imaging Data for Discriminating Pigments and Binders Used in Artworks.Molecules (Basel, Switzerland) · 2022Article
- Performance of High Efficiency Avalanche Poly-SiGe Devices for Photo-Sensing Applications.Sensors (Basel, Switzerland) · 2022Article
- Lifetime-based analysis of binary fluorophores mixtures in the low photon count limit.iScience · 2022Article
- Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution.Nature communications · 2021Article
- Recent innovations in fluorescence lifetime imaging microscopy for biology and medicine.Journal of biomedical optics · 2021Article
- Multispectral fluorescence lifetime imaging device with a silicon avalanche photodetector.Optics express · 2021Article
- Mesoscopic fluorescence lifetime imaging: Fundamental principles, clinical applications and future directions.Journal of biophotonics · 2021Review
- Confocal-based fluorescence fluctuation spectroscopy with a SPAD array detector.Light, science & applications · 2021Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autofluorescence spectroscopy has emerged in recent years as a powerful tool to report label-free contrast between normal and diseased tissues, both in vivo and ex vivo. We report the development of an instrument employing Single Photon Avalanche Diode (SPAD) arrays to realize real-time multispectral autofluorescence lifetime imaging at a macroscopic scale using handheld single-point fibre optic probes, under bright background conditions. At the detection end, the fluorescence signal is passed through a transmission grating and both spectral and temporal information are encoded in the SPAD array. This configuration allows interrogation in the spectral range of interest in real time. Spatial information is provided by an external camera together with a guiding beam that provides a visual reference that is tracked in real-time. Through fast image processing and data analysis, fluorescence lifetime maps are augmented on white light images to provide feedback of the measurements in real-time. We validate and demonstrate the practicality of this technique in the reference fluorophores and in articular cartilage samples mimicking the degradation that occurs in osteoarthritis. Our results demonstrate that SPADs together with fibre probes can offer means to report autofluorescence spectral and lifetime contrast in real-time and thus are suitable candidates for in situ tissue diagnostics.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.