ArticleScientific reports2020
Digital Holographic Multimodal Cross-Sectional Fluorescence and Quantitative Phase Imaging System.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- AI-driven dual-mode phase and label-free fluorescence imaging platform using a single-shot gabor hologram.Microsystems & nanoengineering · 2026Article
- Characterization of a Single-Capture Bright-Field and Off-Axis Digital Holographic Microscope for Biological Applications.Sensors (Basel, Switzerland) · 2025Article
- Dual Field-of-View Off-Axis Spatially Multiplexed Digital Holography Using Fresnel's Bi-Mirror.Sensors (Basel, Switzerland) · 2024Article
- Live Cell Imaging by Single-Shot Common-Path Wide Field-of-View Reflective Digital Holographic Microscope.Sensors (Basel, Switzerland) · 2024Article
- Fluholoscopy-Compact and Simple Platform Combining Fluorescence and Holographic Microscopy.Biosensors · 2023Article
- Label-free optical interferometric microscopy to characterize morphodynamics in living plants.Frontiers in plant science · 2023Review
- Digital Holographic Microscopy to Assess Cell Behavior.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron near Infrared Beam.Biosensors · 2022Article
- Live-dead assay on unlabeled cells using phase imaging with computational specificity.Nature communications · 2022Article
- Lensless Three-Dimensional Quantitative Phase Imaging Using Phase Retrieval Algorithm.Journal of imaging · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a multimodal imaging system based on simple off-axis digital holography, for simultaneous recording and retrieval of cross-sectional fluorescence and quantitative phase imaging of the biological specimen. Synergism in the imaging capabilities can be achieved by incorporating two off-axis digital holographic microscopes integrated to record different information at the same time. The cross-sectional fluorescence imaging is realized by a common-path configuration of the single-shot off-axis incoherent digital holographic system. The quantitative phase imaging, on the other hand, is achieved by another off-axis coherent digital holographic microscopy operating in transmission mode. The fundamental characteristics of the proposed multimodal system are confirmed by performing various experiments on fluorescent beads and fluorescent protein-labeled living cells of the moss Physcomitrella patens lying at different axial depth positions. Furthermore, the cross-sectional live fluorescence and phase imaging of the fluorescent beads are demonstrated by the proposed multimodal system. The experimental results presented here corroborate the feasibility of the proposed system and indicate its potential in the applications to analyze the functional and structural behavior of biological cells and tissues.
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Registered trials
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