ArticleScientific reports2020
Genome-wide screens reveal Escherichia coli genes required for growth of T1-like phage LL5 and V5-like phage LL12.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 22 citations in OpenAlex.
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- Biological Features and In Planta Transcriptomic Analyses of aInternational journal of molecular sciences · 2022Article
- Systematic exploration of Escherichia coli phage-host interactions with the BASEL phage collection.PLoS biology · 2021Article
- Characterization and Food Application of the Novel Lytic Phage BECP10: Specifically Recognizes the O-polysaccharide ofViruses · 2021Article
- Complete Genome Sequence of Escherichia coli Bacteriophage U136B.Microbiology resource announcements · 2021Article
- Trading-off and trading-up in the world of bacteria-phage evolution.Current biology : CB · 2020Article
- High-throughput mapping of the phage resistance landscape in E. coli.PLoS biology · 2020Article
- Isolation and Characterization ofAntibiotics (Basel, Switzerland) · 2020Article
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6 authors at 1 institution in 1 country.
Funding
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Abstract
The host factor requirements of phages and mechanisms of mutational phage insensitivity must be characterized for rational design of phage cocktails. To characterize host dependencies of two novel Escherichia coli phages, the T1-like siphophage LL5 and the V5-like myophage LL12, forward genetic screens were conducted against the Keio collection, a library of single non-essential gene deletions in E. coli str. BW25113. These screens and subsequent experiments identified genes required by phages LL5 and LL12. E. coli mutants deficient in heptose II and the phosphoryl substituent of heptose I of the inner core lipopolysaccharide (LPS) were unable to propagate phage LL5, as were mutants deficient in the outer membrane protein TolC. Mutants lacking glucose I of the LPS outer core failed to propagate LL12. Two additional genes encoding cytoplasmic chaperones, PpiB and SecB, were found to be required for efficient propagation of phage LL5, but not LL12. This screening approach may be useful for identifying host factors dependencies of phages, which would provide valuable information for their potential use as therapeutics and for phage engineering.
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