Evidence map›Paper›PMID 32415096›Full record

ArticleNature communications2020

mRNA display with library of even-distribution reveals cellular interactors of influenza virus NS1.

Yushen Du, Judd F Hultquist, Quan Zhou, Anders Olson, Yenwen Tseng, Tian-Hao Zhang, Mengying Hong, Kejun Tang, Liubo Chen, Xiangzhi Meng and 9 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.0field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Observational
  5. Article
  6. Article
  7. Review
  8. Article
  9. Cleavage and Polyadenylation Specific Factor 1 Promotes Tumor ProgressionFrontiers in cell and developmental biology · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 4 institutions in 2 countries.

Yushen DuDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA. lilyduyushen@g.ucla.edu.ORCID http://orcid.org/0000-0002-8015-4207
Judd F HultquistDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, 94158, USA.
Quan ZhouDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Anders OlsonDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Yenwen TsengDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Tian-Hao ZhangDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Mengying HongCancer Institute, ZJU-UCLA Joint Center for Medical Education and Research, School of Medicine, Zhejiang University, Hangzhou, 310058, China.
Kejun TangCancer Institute, ZJU-UCLA Joint Center for Medical Education and Research, School of Medicine, Zhejiang University, Hangzhou, 310058, China.
Liubo ChenCancer Institute, ZJU-UCLA Joint Center for Medical Education and Research, School of Medicine, Zhejiang University, Hangzhou, 310058, China.
Xiangzhi MengDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Michael J McGregorDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, 94158, USA.
Lei DaiDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Danyang GongDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Laura Martin-SanchoSanford Burnham Prebys Medical Discovery Institute, 10901 North Torrey Pines Road, La Jolla, CA, 92037, USA.
Sumit ChandaSanford Burnham Prebys Medical Discovery Institute, 10901 North Torrey Pines Road, La Jolla, CA, 92037, USA.
Xinming LiDepartment of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, L, Los Angeles, CA, 90095, USA.
Steve BensengerDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.ORCID http://orcid.org/0000-0002-9657-4206
Nevan J KroganDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, 94158, USA.ORCID http://orcid.org/0000-0003-4902-337X
Ren SunDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA. rsun@mednet.ucla.edu.ORCID http://orcid.org/0000-0002-8628-7019
University of California, Los Angeles · USQB3 · USZhejiang University · CNSanford Burnham Prebys Medical Discovery Institute · US

Funding

SARS-CoV adaptations through a Systems Biology Lens (SYBIL)U19AI135972 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI Adolfo Garcia-Sastre · 2018 to 2026
$27.2M
Virology CoreP01CA177322 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SUN, REN · 2014 to 2018
$8.7M
Microbial Pathogenesis and Host DefenseT32AI060537 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Joanne N. Engel · 2004 to 2026
$6.5M
NCI NIH HHS P01 CA177322NIAID NIH HHS U19 AI135972
6 · The paper itself

Abstract

A comprehensive examination of protein-protein interactions (PPIs) is fundamental for the understanding of cellular machineries. However, limitations in current methodologies often prevent the detection of PPIs with low abundance proteins. To overcome this challenge, we develop a mRNA display with library of even-distribution (md-LED) method that facilitates the detection of low abundance binders with high specificity and sensitivity. As a proof-of-principle, we apply md-LED to IAV NS1 protein. Complementary to AP-MS, md-LED enables us to validate previously described PPIs as well as to identify novel NS1 interactors. We show that interacting with FASN allows NS1 to directly regulate the synthesis of cellular fatty acids. We also use md-LED to identify a mutant of NS1, D92Y, results in a loss of interaction with CPSF1. The use of high-throughput sequencing as the readout for md-LED enables sensitive quantification of interactions, ultimately enabling massively parallel experimentation for the investigation of PPIs.

Indexed as

Gene LibraryA549 CellsFatty Acid Synthase, Type IGene OntologyHumansInfluenza A virusInterferonsLipid MetabolismMutationProtein BindingRNA, MessengerViral Nonstructural ProteinsVirus ReplicationFASN protein, humanFatty Acid Synthase, Type IINS1 protein, influenza virusInterferonsRNA, MessengerViral Nonstructural Proteins

Identifiers

PMID32415096
PMCPMC7229031
OpenAlexW3025253491

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.