ArticleMicroorganisms2020
Assessment of ISO Method 15216 to Quantify Hepatitis E Virus in Bottled Water.
Article in Microorganisms, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Assessment of Hepatitis E Virus RNA Detection in Meat Samples: Optimization of Pre-analytical Conditions.Food and environmental virology · 2024Article
- The behaviour ofHeliyon · 2023Article
- SARS-CoV-2 Prevalence in Laparoscopic Surgery Filters. Analysis in Patients with Negative Oropharyngeal RT-qPCR in a Pandemic Context: A Cross-Sectional Study.Journal of personalized medicine · 2021Article
- Occurrence and Accumulation of Human Enteric Viruses and Phages in Process Water from the Fresh Produce Industry.Foods (Basel, Switzerland) · 2021Article
- Enhancement of the Molecular and Serological Assessment of Hepatitis E Virus in Milk Samples.Microorganisms · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Hepatitis E virus (HEV) is one of the causative agents of water-borne human viral hepatitis and considered in Europe an emerging zoonotic pathogen. Analysis of bottled water through a standard method validated for HEV can contribute towards the risk management of this hazard. Putting some recent reports by the European Food Safety Authority in place, this study aimed to assess the performance of the concentration and extraction procedures described in ISO 15216-1:2017 for norovirus and hepatitis A virus on HEV detection. Following the ISO recommendation, the bottled water samples were spiked using serially diluted HEV fecal suspensions together with mengovirus as process control and concentrated by filtration via positively charged nylon membranes. In order to extract viral RNA from the resulting concentrates, two different methods were compared in this study: The one recommended in the ISO norm, NucliSens® MiniMag® system (NS), and an alternative commercially available kit NucleoSpin®RNA virus kit (MN). Finally, three reverse transcription quantitative PCR (RT-qPCR) assays were used to quantify HEV titers. The evaluated procedures resulted in average HEV recoveries of 14.08 ± 4.90% and 3.58 ± 0.30% for the MN and NS methods, respectively. The limit of detection (LoD
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Registered trials
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