Evidence map›Paper›PMID 32413305›Full record

ArticleCurrent biology : CB2020

Tissue Mechanics Regulate Mitotic Nuclear Dynamics during Epithelial Development.

Natalie J Kirkland, Alice C Yuen, Melda Tozluoglu, Nancy Hui, Ewa K Paluch, Yanlan Mao

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current biology : CB, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
2.2field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 32 citations in OpenAlex.

  1. Article
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  5. Reorganizing chromatin by cellular deformation.Current opinion in cell biology · 2024
    Review
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  16. Article
  17. The Mechanics of Mitotic Cell Rounding.Frontiers in cell and developmental biology · 2020
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Natalie J KirklandMRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.
Alice C YuenMRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK; Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
Melda TozluogluMRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.
Nancy HuiMRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.
Ewa K PaluchMRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK; Institute for the Physics of Living Systems, University College London, Gower Street, London WC1E 6BT, UK; Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK. Electronic address: ekp25@cam.ac.uk.
Yanlan MaoMRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK; Institute for the Physics of Living Systems, University College London, Gower Street, London WC1E 6BT, UK. Electronic address: y.mao@ucl.ac.uk.
MRC Laboratory for Molecular Cell Biology · GBUniversity College London · GB

Funding

Medical Research Council MC_U12266BMedical Research Council MC_UU_00012/5Medical Research Council MC_UU_12018/5Medical Research Council MR/L009056/1Medical Research Council MR/T031646/1Wellcome TrustWellcome Trust 103095
6 · The paper itself

Abstract

Cell divisions are essential for tissue growth. In pseudostratified epithelia, where nuclei are staggered across the tissue, each nucleus migrates apically before undergoing mitosis. Successful apical nuclear migration is critical for planar-orientated cell divisions in densely packed epithelia. Most previous investigations have focused on the local cellular mechanisms controlling nuclear migration. Inter-species and inter-organ comparisons of different pseudostratified epithelia suggest global tissue architecture may influence nuclear dynamics, but the underlying mechanisms remain elusive. Here, we use the developing Drosophila wing disc to systematically investigate, in a single epithelial type, how changes in tissue architecture during growth influence mitotic nuclear migration. We observe distinct nuclear dynamics at discrete developmental stages, as epithelial morphology changes. We use genetic and physical perturbations to show a direct effect of cell density on mitotic nuclear positioning. We find Rho kinase and Diaphanous, which facilitate mitotic cell rounding in confined cell conditions, are essential for efficient apical nuclear movement. Perturbation of Diaphanous causes increasing defects in apical nuclear migration as the tissue grows and cell density increases, and these defects can be reversed by acute physical reduction of cell density. Our findings reveal how the mechanical environment imposed on cells within a tissue alters the molecular and cellular mechanisms adopted by single cells for mitosis.

Indexed as

MitosisAnimalsCell NucleusDrosophila melanogasterEpithelial CellsEpitheliumFemaleLarvaMalediaphanousinterkinetic nuclear migrationmitosismitotic roundingpseudostratified epitheliarho kinasetissue mechanics

Identifiers

PMID32413305
PMCPMC7342018
OpenAlexW3025623701

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.