Evidence map›Paper›PMID 32413257›Full record

ArticleACS synthetic biology2020

Design and Experimental Evaluation of a Minimal, Innocuous Watermarking Strategy to Distinguish Near-Identical DNA and RNA Sequences.

Francine J Boonekamp, Sofia Dashko, Donna Duiker, Thies Gehrmann, Marcel van den Broek, Maxime den Ridder, Martin Pabst, Vincent Robert, Thomas Abeel, Eline D Postma and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS synthetic biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Genomic markers on synthetic genomes.Engineering in life sciences · 2021
    Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Francine J BoonekampDepartment of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629HZ Delft, The Netherlands.
Sofia DashkoDepartment of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629HZ Delft, The Netherlands.
Donna DuikerDepartment of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629HZ Delft, The Netherlands.
Thies GehrmannWesterdijk Institute, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
Marcel van den BroekDepartment of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629HZ Delft, The Netherlands.
Maxime den RidderDepartment of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629HZ Delft, The Netherlands.
Martin PabstDepartment of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629HZ Delft, The Netherlands.
Vincent RobertWesterdijk Institute, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
Thomas AbeelIntelligent Systems - Delft Bioinformatics Lab, Delft University of Technology, Van Mourik Broekmanweg 6, 2628XE Delft, The Netherlands.
Eline D PostmaDepartment of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629HZ Delft, The Netherlands.
Jean-Marc DaranDepartment of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629HZ Delft, The Netherlands.
Pascale Daran-LapujadeDepartment of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629HZ Delft, The Netherlands.ORCID 0000-0002-4097-7831
Delft University of Technology · NLWesterdijk Fungal Biodiversity Institute · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The construction of powerful cell factories requires intensive and extensive remodelling of microbial genomes. Considering the rapidly increasing number of these synthetic biology endeavors, there is an increasing need for DNA watermarking strategies that enable the discrimination between synthetic and native gene copies. While it is well documented that codon usage can affect translation, and most likely mRNA stability in eukaryotes, remarkably few quantitative studies explore the impact of watermarking on transcription, protein expression, and physiology in the popular model and industrial yeast

Indexed as

Base SequenceCRISPR-Cas SystemsDNAGene EditingGlycolysisResearch DesignRNASaccharomyces cerevisiaeSynthetic BiologyDNARNAdifferential RNA expression analysisDNA and RNA watermarksgenome engineeringglycolysispathway swappingSaccharomyces cerevisiae

Identifiers

PMID32413257
PMCPMC7309318
OpenAlexW3024732647

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.