ArticleLight, science & applications2020
Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo.
Article in Light, science & applications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 92 citations in OpenAlex.
- Two-Photon Fluorescence and Second-Harmonic Generation Microscopy: Principles and Biomedical Applications.Journal of biophotonics · 2026Review
- Investigating the Utility of Fractal Measures as a Diagnostic for Glaucoma.Statistics in medicine · 2026Article
- The Eye and the Brain: Photonic Devices in Neuro-Ophthalmology.Diseases (Basel, Switzerland) · 2026Review
- Physics-informed multi-encoder adaptive optics enables rapid aberration correction for intravital microscopy of deep complex tissue.Nature communications · 2026Article
- A Three-dimensional Analytical Framework for Retinal Microvasculature Reveals Layer-associated Vulnerability in Development and Neovascular Remodeling.bioRxiv : the preprint server for biology · 2026Article
- Structure and function of mouse lens suture examined by 2-photon fluorescence microscopic imaging.Scientific reports · 2026Article
- Refractive adaptive optics scanning light ophthalmoscope with fast 2D MEMS scanner.Biomedical optics express · 2026Article
- Focusing optical phased array for tissue interrogation with improved side-lobe suppression and simplified beam steering.Biomedical optics express · 2026Article
- Characterization of adeno-associated viral transduction of retinal ganglion cells in adult and old mice.Molecular therapy. Methods & clinical development · 2025Article
- Mechanisms of action of retinal microglia in diabetic retinopathy (Review).International journal of molecular medicine · 2025Review
- Descattering and image restoration with a transformer-based neural network in deep tissue imaging.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Transpupillary in vivo two-photon imaging reveals enhanced surveillance of retinal microglia in diabetic mice.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Telecentric model eye for correction of image distortion in adaptive optics ophthalmoscopes.Biomedical optics express · 2025Article
- High-resolution multimodal visible light optical coherence tomography and scanning laser ophthalmoscopy forBiomedical optics express · 2025Article
- Imaging 3D cell cultures with optical microscopy.Nature methods · 2025Review
- Quantitative fs-laser crystalline lens softening surgery towards presbyopia treatment based on optical coherence elastography.Biomedical optics express · 2025Article
- Deep-Penetrating and High-Resolution Continuous-Wave Nonlinear Microscopy Based on Homologous Dual-Emission Upconversion Adaptive Optics.Nano letters · 2025Article
- Principles of microscopy for ophthalmologists.Eye (London, England) · 2025Review
- Viscocohesive hyaluronan gel enhances stability of intravital multiphoton imaging with subcellular resolution.Neurophotonics · 2025Article
- Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
In vivo fundus imaging offers non-invasive access to neuron structures and biochemical processes in the retina. However, optical aberrations of the eye degrade the imaging resolution and prevent visualization of subcellular retinal structures. We developed an adaptive optics two-photon excitation fluorescence microscopy (AO-TPEFM) system to correct ocular aberrations based on a nonlinear fluorescent guide star and achieved subcellular resolution for in vivo fluorescence imaging of the mouse retina. With accurate wavefront sensing and rapid aberration correction, AO-TPEFM permits structural and functional imaging of the mouse retina with submicron resolution. Specifically, simultaneous functional calcium imaging of neuronal somas and dendrites was demonstrated. Moreover, the time-lapse morphological alteration and dynamics of microglia were characterized in a mouse model of retinal disorder. In addition, precise laser axotomy was achieved, and degeneration of retinal nerve fibres was studied. This high-resolution AO-TPEFM is a promising tool for non-invasive retinal imaging and can facilitate the understanding of a variety of eye diseases as well as neurodegenerative disorders in the central nervous system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.