Evidence map›Paper›PMID 32411108›Full record

ArticleFrontiers in microbiology2020

Crystal Structure and Active Site Engineering of a Halophilic γ-Carbonic Anhydrase.

Malvina Vogler, Ram Karan, Dominik Renn, Alexandra Vancea, Marie-Theres Vielberg, Stefan W Grötzinger, Priya DasSarma, Shiladitya DasSarma, Jörg Eppinger, Michael Groll and 1 more

Open access · goldAbstract read
In one paragraph

Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 26 citations in OpenAlex.

  1. Article
  2. Introduction to Carbonic Anhydrase.Sub-cellular biochemistry · 2026
    Review
  3. Article
  4. Alpha Carbonic Anhydrase fromInternational journal of molecular sciences · 2024
    Article
  5. Article
  6. Article
  7. Industrial Biotechnology Based on Enzymes From Extreme Environments.Frontiers in bioengineering and biotechnology · 2022
    Review
  8. Article
  9. Article
  10. Review
  11. Review
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 3 countries.

Malvina VoglerKAUST Catalysis Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
Ram KaranKAUST Catalysis Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
Dominik RennKAUST Catalysis Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
Alexandra VanceaKAUST Catalysis Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
Marie-Theres VielbergCenter for Integrated Protein Science Munich, Department of Chemistry, Technische Universität München, Garching, Germany.
Stefan W GrötzingerKAUST Catalysis Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
Priya DasSarmaDepartment of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland School of Medicine, Baltimore, MD, United States.
Shiladitya DasSarmaDepartment of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland School of Medicine, Baltimore, MD, United States.
Jörg EppingerKAUST Catalysis Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
Michael GrollCenter for Integrated Protein Science Munich, Department of Chemistry, Technische Universität München, Garching, Germany.
Magnus RuepingKAUST Catalysis Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
King Abdullah University of Science and Technology · SACenter for Integrated Protein Science Munich · DEUniversity of Maryland, Baltimore · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Environments previously thought to be uninhabitable offer a tremendous wealth of unexplored microorganisms and enzymes. In this paper, we present the discovery and characterization of a novel γ-carbonic anhydrase (γ-CA) from the polyextreme Red Sea brine pool Discovery Deep (2141 m depth, 44.8°C, 26.2% salt) by single-cell genome sequencing. The extensive analysis of the selected gene helps demonstrate the potential of this culture-independent method. The enzyme was expressed in the bioengineered haloarchaeon

Indexed as

extremophilesextremozymegamma-carbonic anhydrasehalophilesmutagenesissalt adaptationthermophiles

Identifiers

PMID32411108
PMCPMC7199487
OpenAlexW3022543080

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.