Evidence map›Paper›PMID 32410727›Full record

ArticleParasite (Paris, France)2020

Molecular identification and subtyping of Blastocystis sp. in laboratory rats in China.

Junqiang Li, Yueyue Yuan, Yuxi Jiang, Wen Wang, Liqin Chao, Ruiqin Sun, Jun Li, Md Robiul Karim, Meng Qi

Open access · goldAbstract read
In one paragraph

Article in Parasite (Paris, France), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
0.8field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 10 citations in OpenAlex.

  1. Pooled it
  2. Predominance of theOne health (Amsterdam, Netherlands) · 2024
    Article
  3. Article
  4. Article
  5. Rare Occurrence ofJournal of clinical medicine · 2022
    Article
  6. Article
  7. The Coexistence ofBiology · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Junqiang LiAcademy of Chinese Medical Sciences, Henan University of Chinese Medicine, 450046 Zhengzhou, PR China.
Yueyue YuanAcademy of Chinese Medical Sciences, Henan University of Chinese Medicine, 450046 Zhengzhou, PR China.
Yuxi JiangCollege of Animal Science, Tarim University, Alar, 843300 Xinjiang, PR China.
Wen WangCollege of Animal Science, Tarim University, Alar, 843300 Xinjiang, PR China.
Liqin ChaoAcademy of Chinese Medical Sciences, Henan University of Chinese Medicine, 450046 Zhengzhou, PR China.
Ruiqin SunAcademy of Chinese Medical Sciences, Henan University of Chinese Medicine, 450046 Zhengzhou, PR China.
Jun LiAcademy of Chinese Medical Sciences, Henan University of Chinese Medicine, 450046 Zhengzhou, PR China.
Md Robiul KarimFaculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, 1706 Gazipur, Bangladesh.
Meng QiCollege of Animal Science, Tarim University, Alar, 843300 Xinjiang, PR China.
First Affiliated Hospital of Henan University · CNTarim University · CNBangabandhu Sheikh Mujibur Rahman Agricultural University · BD

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Blastocystis sp. is a ubiquitous protist that has been frequently reported in humans and animals worldwide. A total of 355 fecal samples of experimental rats were collected from four laboratory rearing facilities in China, and Blastocystis sp. was detected by PCR amplification of the partial small subunit ribosomal (SSU) rRNA gene. Twenty-nine (8.2%, 29/355) samples were positive for Blastocystis sp., with the highest infection rate (20.7%, 24/116) in rats of the Zhengzhou1, followed by that in the Zhengzhou2 (5.0%, 2/40), Shenyang (3.0%, 3/100) and Wuhan (0) rearing facilities. Among the three rat strains, Sprague-Dawley (SD) rats had higher infection rates (11.3%, 17/151) compared to Wistar rats (8.7%, 9/104) and spontaneously hypertensive (SH) rats (3.0%, 3/100). Two Blastocystis sp. subtypes (ST4 and ST7) were identified. ST4 was the predominant subtype detected in 26 samples (89.7%). A phylogenetic analysis demonstrated that the sequences of ST4 and ST7 obtained in this study were clustered with their reference subtypes. To our knowledge, this is the first report of Blastocystis sp. in experimental rats in China. Pathogen infections in laboratory animals need to be monitored due to fecal-oral transmission.

Indexed as

Genetic VariationPhylogenyAnimalsAnimals, LaboratoryBlastocystisBlastocystis InfectionsChinaDNA, ProtozoanDNA, RibosomalFecesFemaleRatsRats, Inbred SHRRats, Sprague-DawleyRats, WistarDNA, ProtozoanDNA, RibosomalBlastocystis sp.IdentificationLaboratory ratsSubtype

Identifiers

PMID32410727
PMCPMC7227369
OpenAlexW3024818621

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.