ArticleParasites & vectors2020
Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 15 citations in OpenAlex.
- Viability of Trypanosoma cruzi in Triatoma pallidipennis Post-mortem.Acta parasitologica · 2026Article
- Blechomonas campbelli from Ctenocephalides felis: in vitro development, thermotolerance, and phylogenetic insights.Memorias do Instituto Oswaldo Cruz · 2026Article
- Exploring a gene co-expression network throughout the trypanosoma cruzi life cycle.BMC genomics · 2025Article
- P21 Ablation Unveils Strain-Specific Transcriptional Reprogramming inInternational journal of microbiology · 2025Article
- Metacyclogenesis as the Starting Point of Chagas Disease.International journal of molecular sciences · 2023Review
- Differential expression of meiosis and homologous recombination-related genes in the life cycle of Trypanosoma cruzi.Parasitology research · 2023Article
- Transcriptomic analysis of benznidazole-resistant and susceptible Trypanosoma cruzi populations.Parasites & vectors · 2023Article
- Transcriptomic analysis of the adaptation to prolonged starvation of the insect-dwellingFrontiers in cellular and infection microbiology · 2023Article
- The endoplasmic reticulum of trypanosomatids: An unrevealed road for chemotherapy.Frontiers in cellular and infection microbiology · 2022Review
- Review
- AParasitology · 2021Article
- Higher temperatures reduce the number of Trypanosoma cruzi parasites in the vector Triatoma pallidipennis.Parasites & vectors · 2021Article
- Stress Induces Release of Extracellular Vesicles byJournal of immunology research · 2021Article
- Different Transcriptomic Responses After Moderate and Severe Heat Shock in Trypanosoma cruzi Epimastigotes.The Journal of eukaryotic microbiologyArticle
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundSevere changes in temperature can affect the behavior and ecology of some infectious agents. Trypanosoma cruzi is a protozoan that causes Chagas disease. This parasite has high genetic variability and can be divided into six discrete typing units (DTUs). Trypanosoma cruzi also has a complex life-cycle, which includes the process of metacyclogenesis when non-infective epimastigote forms are differentiated into infective metacyclic trypomastigotes (MT). Studies in triatomines have shown that changes in temperature also affect the number and viability of MT.
methodsThe objective of this study was to evaluate how temperature affects the transcriptional profiles of T. cruzi I and II (TcI and TcII) MT by exposing parasites to two temperatures (27 °C and 28 °C) and comparing those to normal culture conditions at 26 °C. Subsequently, RNA-seq was conducted and differentially expressed genes were quantified and associated to metabolic pathways.
resultsA statistically significant difference was observed in the number of MT between the temperatures evaluated and the control, TcII DTU was not strongly affected to exposure to high temperatures compared to TcI. Similar results were found when we analyzed gene expression in this DTU, with the greatest number of differentially expressed genes being observed at 28 °C, which could indicate a dysregulation of different signaling pathways under this temperature. Chromosome analysis indicated that chromosome 1 harbored the highest number of changes for both DTUs for all thermal treatments. Finally, gene ontology (GO) analyses showed a decrease in the coding RNAs involved in the regulation of processes related to the metabolism of lipids and carbohydrates, the evasion of oxidative stress, and proteolysis and phosphorylation processes, and a decrease in RNAs coding to ribosomal proteins in TcI and TcII, along with an increase in the expression of surface metalloprotease GP63 in TcII.
conclusionsSlight temperature shifts lead to increased cell death of metacyclic trypomastigotes because of the deregulation of gene expression of different processes essential for the TcI and TcII DTUs of T. cruzi.
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