ArticleBMC microbiology2020
In vitro synergistic potentials of novel antibacterial combination therapies against Salmonella enterica serovar Typhimurium.
Article in BMC microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Exploring the effects of phenolic compounds and essential oils in poultry: A sustainable strategy to combat Salmonella biofilm infections.Poultry science · 2025Review
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- Research Progress on the Antibacterial Activity of Natural Flavonoids.Antibiotics (Basel, Switzerland) · 2025Review
- Phytochemical Analysis and Antimicrobial Activity ofMicroorganisms · 2024Article
- Plant-Derived Phenolic Acids Limit the Pathogenesis ofMolecules (Basel, Switzerland) · 2024Article
- An update on the potential mechanism of gallic acid as an antibacterial and anticancer agent.Food science & nutrition · 2023Review
- Cinnamaldehyde Restores Ceftriaxone Susceptibility against Multidrug-ResistantInternational journal of molecular sciences · 2023Article
- Article
- Efficacy of Acidified Oils againstApplied and environmental microbiology · 2022Article
- Lysophosphatidylcholine Enhances Bactericidal Activity by Promoting Phagosome Maturation via the Activation of the NF-κB Pathway duringMolecules and cells · 2020Article
- Synergism of the Combination of Traditional Antibiotics and Novel Phenolic Compounds againstPathogens (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe antibiotics generally used in farm animals are rapidly losing their effectiveness all over the world as bacteria develop antibiotic resistance. Like some other pathogenic bacteria multidrug-resistant strains of Salmonella enterica serovar Typhimurium (S. Typhimurium) are also frequently found in animals and humans which poses a major public health concern. New strategies are needed to block the development of resistance and to prolong the life of traditional antibiotics. Thus, this study aimed to increase the efficacy of existing antibiotics against S. Typhimurium by combining them with opportunistic phenolic compounds gallic acid (GA), epicatechin, epicatechin gallate, epigallocatechin and hamamelitannin. Fractional inhibitory concentration indexes (FICI) of phenolic compound-antibiotic combinations against S. Typhimurium were determined. Based on the FICI and clinical importance, 1 combination (GA and ceftiofur) was selected for evaluating its effects on the virulence factors of this bacterium. Viability of Rattus norvegicus (IEC-6) cell in presence of this antibacterial combination was evaluated.
resultsMinimum inhibitory concentrations (MICs) of GA, epigallocatechin and hamamelitannin found against different strains of S. Typhimurium were 256, (512-1024), and (512-1024) μg/mL, respectively. Synergistic antibacterial effect was obtained from the combination of erythromycin-epicatechin gallate (FICI: 0.50) against S. Typhimurium. Moreover, additive effects (FICI: 0.502-0.750) were obtained from 16 combinations against this bacterium. The time-kill assay and ultrastructural morphology showed that GA-ceftiofur combination more efficiently inhibited the growth of S. Typhimurium compared to individual antimicrobials. Biofilm viability, and swimming and swarming motilities of S. Typhimurium in presence of GA-ceftiofur combination were more competently inhibited than individual antimicrobials. Viabilities of IEC-6 cells were more significantly enhanced by GA-ceftiofur combinations than these antibacterials alone.
conclusionsThis study suggests that GA-ceftiofur combination can be potential medication to treat S. Typhimurium-associated diarrhea and prevent S. Typhimurium-associated blood-stream infections (e.g.: fever) in farm animals, and ultimately its transmission from animal to human. Further in vivo study to confirm these effects and safety profiles in farm animal should be undertaken for establishing these combinations as medications.
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