ArticleAntonie van Leeuwenhoek2020
Mucin pre-cultivated Lactobacillus reuteri E shows enhanced adhesion and increases mucin expression in HT-29 cells.
Article in Antonie van Leeuwenhoek, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Effects of commercial direct-fed microbials and zinc-methionine supplements on growth performance in preweaning Holstein calves under commercial rearing conditions.Veterinary and animal science · 2026Article
- Investigation of Oral Toxicity Mechanisms of Low-Molecular-Weight Polycyclic Aromatic Hydrocarbons and Probiotic Interventions Under the Framework of the "Gut-Lung Axis".Foods (Basel, Switzerland) · 2026Review
- Complete Genome Sequence Analysis, Probiotic Properties, and Safety Assessment of Healthy Human-Derived Pediococcus pentosaceus.Probiotics and antimicrobial proteins · 2026Article
- Studies on the Probiotic, Adhesion, and Induction Properties of Artisanal Lactic Acid Bacteria: to Customize a Gastrointestinal Niche to Trigger Anti-obesity Functions.Probiotics and antimicrobial proteins · 2025Article
- Prophylactic and therapeutic effects of Limosilactobacillus reuteri E on 5-fluorouracil-induced intestinal mucositis in Caco-2 cells.Folia microbiologica · 2025Article
- Mechanism of probiotics in the intervention of colorectal cancer: a review.World journal of microbiology & biotechnology · 2024Review
- Development of an intestinal epithelial cell line and organoids derived from the same swine and characterization of their antiviral responses.Bioscience of microbiota, food and health · 2024Article
- Anti-Antibiotics (Basel, Switzerland) · 2023Article
- Multilayer Coating with Red Ginseng Dietary Fiber Improves Intestinal Adhesion and Proliferation of Probiotics in Human Intestinal Epithelial Models.Journal of microbiology and biotechnology · 2023Article
- The role of potential probiotic strainsFrontiers in microbiology · 2023Review
- Assessment of the safety and probiotic properties ofFrontiers in microbiology · 2022Article
- Lactic Acid Bacteria Exert a Hepatoprotective Effect against Ethanol-Induced Liver Injury in HepG2 Cells.Microorganisms · 2021Article
- Article
- Mutual Preservation: A Review of Interactions Between Cervicovaginal Mucus and Microbiota.Frontiers in cellular and infection microbiology · 2021Review
- Microbiome Analysis Reveals the Attenuation Effect ofFrontiers in cellular and infection microbiology · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Adhesion of probiotic bacteria to the mucus layer lining the gastrointestinal tract is necessary for its effective colonisation and specific therapeutic effects. Enrichment of growth medium with mucin might stimulate bacterial adhesion, probably by increasing the expression of surface structures responsible for bacteria-gut epithelia and/or mucus interactions. The aim of this study was to determine if pre-cultivation of potentially probiotic strain Lactobacillus reuteri E (LRE) with mucin stimulates its adherence to colon cell line HT-29 and if the increased adhesion modulates mucin expression in these cells. The mucin-producing HT-29 cell line was co-cultivated for 2 h with LRE grown in MRS broth or MRS broth enriched with pig gastric mucin (LRE + M). The adherence ability of LRE was evaluated microscopically and by plate counting. The relative gene expression was measured by qPCR. Pre-cultivation of LRE in mucin enriched medium significantly increased its adhesion to 14 days HT-29 in comparison with LRE by both methods (28.64% vs. 23.83%, evaluated microscopically, and 14.31 ± 3.95 × 10
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